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1. (NZ545936) Method for linking sequences of interest

Office : New Zealand
Application Number: 545936 Application Date: 17.09.2004
Publication Number: 545936 Publication Date: 30.04.2009
Grant Number: Grant Date: 13.08.2009
Publication Kind : B
Prior PCT appl.: Application Number:PCTDK2004000633 ; Publication Number: Click to see the data
IPC:
C12N 15/10
C12Q 1/68
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants: Symphogen A/S
Inventors: Oleksiewicz, Martin B
Nielsen, Lars S
Andersen, Peter S
Hansen, Margit H
Agents: BALDWINS INTELLECTUAL PROPERTY
Priority Data: 03 01867 17.12.2003 DK
04 00782 15.05.2004 DK
03 504455 18.09.2003 US
03 504589 18.09.2003 US
Title: (EN) Method for linking sequences of interest
Abstract:
(EN) Patent 545936 Disclosed is a method for linking a plurality of non-contiguous nucleotide sequences of interest comprising: amplifying, in a multiplex molecular amplification procedure, nucleotide sequences that comprise variable regioin encoding sequencesusing a template derived from an isolated single cell; and effecting linkage of the amplified sequences to form a cognate pair of variable encoding sequences. The disclosure provides an efficient method of linking two or more nucleotide sequences encoding for domains or subunits of a heteromeric protein, in a single reaction. Especially, the linkage of variable region encoding sequences from e.g. immunoglobulins, T cell receptors or B cell receptors is eased. The disclosed method allows for a more efficient way of generating libraries of variable region encoding sequences. The capability to perform the multiplex overlap-extension RT-PCR using template derived from an isolated single cell enables the generation of cognate pair libraries in a high-throughput format...
Also published as:
IS8415NO20061373MXPA/a/2006/002894KR1020060092218ZA2006/03032IL173688
EP1670912EP1921144JP2007505611RU02392324US20070141048JP4651619
CN1856571CA2539576DK1670912AU2004286019IN891/KOLNP/2006WO/2005/042774