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1. NZ524033 - Compositions and methods for detecting target microorganisms in a sample

Office
New Zealand
Application Number 524033
Application Date 05.02.2003
Publication Number 524033
Publication Date 27.06.2008
Grant Number 524033
Grant Date 09.10.2008
Publication Kind B
IPC
C12Q 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
C12Q 1/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C40B 50/08
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
50Methods of creating libraries, e.g. combinatorial synthesis
08Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
H04N 5/31
HELECTRICITY
04ELECTRIC COMMUNICATION TECHNIQUE
NPICTORIAL COMMUNICATION, e.g. TELEVISION
5Details of television systems
30Transforming light or analogous information into electric information
31Transforming ultrasonic, sonic or infrasonic radiation
CPC
C12Q 1/045
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
045Culture media therefor
Applicants BIOCONTROL SYSTEMS, INC.
Inventors Townsend, David
Agents AJ PARK
Priority Data 00 228956 28.08.2000 US
Title
(EN) Compositions and methods for detecting target microorganisms in a sample
Abstract
(EN)
Patent 524033 A composition for detecting a target microorganism in a sample, comprising a conditionally detectable marker, wherein said marker is capable of providing a detectable signal when in contact with a viable microorganism, and a substrate for an enzyme that is substantially absent from the target microorganism. Said target microorganism is selected from bacteria, yeast, mold, fungi, protozoa or viruses. Also disclosed is a medium for detecting viable microorganisms in a sample comprising: a) a substrate for an aminopeptidase; b) a conditionally detectable marker, wherein said marker is capable of providing a detectable signal when in contact with a viable moicroorganism; c) a signal moiety linked to the substrate, said moiety providing a detectable signal when cleaved by said aminopeptidase from said microorganism; d) a growth supporting medium for target or non-target microorganism. and a method for using said medium for detecting viable microorganisms in a sample, comprising: a) providing said medium; b) inoculating said medium with the sample to be tested; c) incubating the inoculated medium under suitable conditions wherein the enzyme substrate is capable of being acted upon by an enzyme from substantially all non-target microorganisms to produce a detectable signal; and d) comparing the difference between the signal generated by conditionally detectable marker and the enzyme substrate, whereby the absence or decrease in a detectable signal indicates the presence of target microorganisms in the sample.