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1. (KR1020060092218) METHOD FOR LINKING SEQUENCES OF INTEREST

Office : Republic of Korea
Application Number: 1020067005488 Application Date: 18.03.2006
Publication Number: 1020060092218 Publication Date: 22.08.2006
Publication Kind : A
IPC:
C12N 15/10
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
CPC:
C12N 15/1093
C07K 16/00
C07K 16/005
C07K 16/1282
C07K2317/21
C07K2317/55
C12N 15/1096
C40B 40/08
Applicants: SYMPHOGEN A/S
심포젠 에이/에스
Inventors: OLEKSIEWICZ MARTIN B.
올렉지비츠, 마르틴, 베.
NIELSEN LARS S.
니일센, 라르스, 에스.
ANDERSEN PETER S.
안데르센, 페터, 에스.
HANSEN MARGIT H.
한센, 마르짓, 하.
Agents: 남상선
Priority Data: PA 2003 01867 17.12.2003 DK
PA 2004 00782 15.05.2004 DK
60/504,455 18.09.2003 US
60/504,589 18.09.2003 US
Title: (EN) METHOD FOR LINKING SEQUENCES OF INTEREST
(KO) 관심있는 서열을 결합시키는 방법
Abstract: front page image
(EN)

Multiplex overlap-extension RT-PCR provides an efficient method of linking two or more nucleotide sequences encoding for domains or subunits of a heteromeric protein, in a single reaction. Especially, the linkage of variable region encoding sequences from e.g. immunoglobulins, T cell receptors or B cell receptors is eased with the method of the present invention. This allows for a more efficient way of generating libraries of variable region encoding sequences. The capability to perform the multiplex overlap-extension RT- PCR using template derived from an isolated single cell enables the generation of cognate pair libraries in a high-throughput format.

© KIPO & WIPO 2007


(KO) 복합 중복-신장 RT-PCR은 이량체 단백질의 도메인 또는 서브유닛을 엔코딩하는 둘 이상의 누클레오티드 서열을 단일 반응으로 결합시키는 효율적인 방법을 제공한다. 특히, 면역글로불린, T 세포 수용체 또는 B 세포 수용체로부터의 가변 영역 엔코딩 서열이 본 발명의 방법으로 용이하게 결합된다. 이는 가변 영역 엔코딩 서열의 라이브러리를 생성시키는 보다 효율적인 방법을 가능하게 한다. 단리된 단일 세포로부터 유래된 주형을 이용한 복합 중복-신장 RT-PCR의 수행능은 고처리량 형식으로 동족 쌍 라이브러리의 생성을 가능하게 한다.
Also published as:
IS8415NO20061373MXPA/a/2006/002894ZA2006/03032IL173688EP1670912
EP1921144JP2007505611RU02392324US20070141048JP4651619CN1856571
CA2539576NZ545936DK1670912AU2004286019IN891/KOLNP/2006WO/2005/042774