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1. KR1020050043978 - PROCESS FOR PRODUCING PURINE NUCLEOSIDES VIA FERMENTATION

Office
Republic of Korea
Application Number 1020057004750
Application Date 18.03.2005
Publication Number 1020050043978
Publication Date 11.05.2005
Grant Number 100697551
Grant Date 21.03.2007
Publication Kind B1
IPC
C12P 19/32
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
32having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same-ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
C12N 1/21
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
Applicants AJINOMOTO CO., INC.
아지노모토 가부시키가이샤
Inventors MATSUI HIROSHI
마쓰이 히로시
KAWASAKI HISASHI
가와사키 히사시
SHIMAOKA MEGUMI
시마오카 메구미
TAKENAKA YASUHIRO
다케나카 야스히로
KURAHASHI OSAMU
구라하시 오사무
Agents 이병호
Priority Data JP-P-1997-00194603 18.07.1997 JP
Title
(EN) PROCESS FOR PRODUCING PURINE NUCLEOSIDES VIA FERMENTATION
(KO) 발효에 의한 퓨린 뉴클레오사이드의 제조 방법
Abstract
(EN)

A process for producing purine nucleosides which comprises breeding and culturing microorganisms having a gene encoding an enzyme wherein the feedback inhibition has been substantially relieved by substituting one or two amino acid residues in PRPP amidotransferase encoded by Escherichia coli purF, a gene encoding a protein wherein the repressor in the purine nucleotide biosynthesis system encoded by purR has been inactivated and a gene encoding an enzyme wherein purine nucleoside phosphorylase encoded by deoD has been inactivated, a gene encoding an enzyme wherein succinyl-AMP synthase encoded by purA has been inactivated, a gene encoding an enzyme wherein 6-phosphogluconate dehydratase encoded by edd has been inactivated and a gene encoding an enzyme wherein phosphoglucose isomerase encoded by pgi has been inactivated, or the like.

© KIPO & WIPO 2007


(KO) 본 발명은 에스케리키아 콜라이 purF에 의해 암호화되는 PRPP 아미도트랜스퍼라제 중 아미노산 잔기 1개 또는 2개를 치환시킴으로써 피드백 억제가 실질적으로 해제된 효소를 암호화하는 유전자, purR에 의해 암호화된 퓨린 뉴클레오티드 생합성계중 리프레서가 불활성화된 단백질을 암호화하는 유전자, 및 deoD에 의해 암호화되는 퓨린 뉴클레오사이드 포스포릴라제가 불활성화된 효소를 암호화하는 유전자, purA에 의해 암호화된 석시닐-AMP 신타제가 불활성화된 효소를 암호화하는 유전자, edd에 의해 암호화된 6-포스포글루코네이트 데하이드라타제가 불활성화된 효소를 암호화하는 유전자 및 pgi에 의해 암호화되는 포스포글루코스 이소머라제가 불활성화된 효소를 암호화하는 유전자 등을 갖는 미생물을 육종하여 배양시킴을 포함하는 퓨린 뉴클레오사이드의 제조 방법에 관한 것이다.