KR1020220029579 진균 포자의 형질전환 방법

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Republic of Korea

Application Number 1020217042011

Application Date 17.06.2020

Publication Number 1020220029579

Publication Date 08.03.2022

Publication Kind A

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Applicants 바스프 플랜트 사이언스 컴퍼니 게엠베하

Inventors 슈투이퍼, 마르텐
샤흐챠벨, 도렌
호프, 비르기트
멘첼, 토비아스
지페, 이자벨라
티네스, 에카르트
야코프, 슈테판
베커, 미하엘
위에멜린, 알렉산더

Agents 장덕순
이귀동

Priority Data 19182791.4 27.06.2019 EP
19217396.1 18.12.2019 EP

Title

(KO) 진균 포자의 형질전환 방법

Abstract

(KO) 본 발명은 진균 포자 및 폴리뉴클레오티드를 보유하는 자기 나노입자의 혼합물을 자석 및/또는 자기장에 노출시키는 것을 포함하는, 폴리뉴클레오티드 분자를 진균 포자 내로 도입하는 방법을 제공한다. 본 발명은 또한 본원에 개시된 방법 단계의 단계를 포함하고, 상기 폴리뉴클레오티드 분자를 상기 포자의 게놈 내로 통합시켜 포자를 형질전환시키는 진균 형질전환 방법을 제공한다. 본 발명은 또한 핵산이 로딩된 자기 나노입자를 포함하는 진균 포자에 핵산을 전달하기 위한 시스템, 상기 폴리뉴클레오티드가 로딩된 MNP 및 진균 포자를 포함하는 폴리뉴클레오티드로 진균 포자를 형질전환시키기 위한 키트 뿐만 아니라 핵산 분자가 로딩된 MNP 및 진균 포자를 포함하는 조성물을 제공한다.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79	Vectors or expression systems specially adapted for eukaryotic hosts
80	for fungi

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
13	Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
87	Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79	Vectors or expression systems specially adapted for eukaryotic hosts
80	for fungi

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
13	Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

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1. KR1020220029579 - 진균 포자의 형질전환 방법