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1. (JP2012162542) PROCESS FOR PRODUCING SUGAR CHAIN DERIVATIVE, STRUCTURE ANALYSIS METHOD, AND SUGAR CHAIN DERIVATIVE

Office : Japan
Application Number: 2012077958 Application Date: 29.03.2012
Publication Number: 2012162542 Publication Date: 30.08.2012
Publication Kind : A
IPC:
C07H 3/06
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
3
Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
06
Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
CPC:
G01N 30/72
C07H 3/06
C08B 37/006
G01N 30/84
G01N2030/8429
Y10T 436/143333
Applicants: OTSUKA CHEM CO LTD
大塚化学株式会社
Inventors: KAKEHI KAZUAKI
掛樋 一晃
KINOSHITA MITSUHIRO
木下 充弘
MATSUNO HIROKI
松野 裕樹
Agents: 南条 雅裕
松崎 隆
Priority Data: 2005209077 19.07.2005 JP
Title: (EN) PROCESS FOR PRODUCING SUGAR CHAIN DERIVATIVE, STRUCTURE ANALYSIS METHOD, AND SUGAR CHAIN DERIVATIVE
(JA) 糖鎖誘導体の製造方法、構造解析方法、及び糖鎖誘導体
Abstract:
(EN)

PROBLEM TO BE SOLVED: To provide a process for preparing an oligosaccharide derivative from an oligosaccharide mixture.

SOLUTION: The process for preparing the oligosaccharide derivative includes the steps of: (a) introducing a lipophilic group into oligosaccharides of the mixture to obtain a mixture of oligosaccharide derivatives; (b) treating the oligosaccharide derivative mixture by serotonin affinity column chromatography; (c) conducting normal phase chromatography with use of an amino column or amide column after the step (b); and (d) treating the resulting eluate with a glycosidase before the step (c).

COPYRIGHT: (C)2012,JPO&INPIT


(JA)

【課題】糖鎖混合物から糖鎖誘導体を製造する方法の提供。
【解決手段】(a)糖鎖混合物中の糖鎖に脂溶性基を導入して糖鎖誘導体の混合物を得る工程、(b)該糖鎖誘導体の混合物をセロトニンアフィニティーカラムクロマトグラフィーで処理する工程、(c)(b)工程の後にアミノカラム又はアミドカラムを用いる順相クロマトグラフィーで処理する工程、および(d)(c)工程の前に糖加水分解酵素で処理する工程を備えた糖鎖誘導体の製造方法。
【選択図】なし


Also published as:
KR1020080028994SG149832KR1020100029154EP1908783JPWO2007011055US20090215100
US20130074585CN101223194CN101949901CN102516402CA2616065CA2759070
KR1020100092042AU2006270732AU2010202058WO/2007/011055