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1. (JP4651619) 目的とする配列を連結するための方法

Office : Japan
Application Number: 2006526523T Application Date: 17.09.2004
Publication Number: 4651619 Publication Date: 16.03.2011
Grant Number: 4651619 Grant Date: 16.03.2011
Publication Kind : B2
Prior PCT appl.: Application Number:WODK2004000633 ; Publication Number: Click to see the data
IPC:
C12N 15/9
C07K 16/0
C07K 16/12
C12N 5/10
C12N 15/10
C12N 15/13
C12P 21/2
C12P 21/8
C40B 40/8
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
12
against material from bacteria
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
12
Genes encoding animal proteins
13
Immunoglobulins
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
08
Monoclonal antibodies
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40
Libraries per se, e.g. arrays, mixtures
04
Libraries containing only organic compounds
06
Libraries containing nucleotides or polynucleotides, or derivatives thereof
08
Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
CPC:
C07K 16/00
C07K 16/005
C07K 16/1282
C07K2317/21
C07K2317/55
C12N 15/1093
C12N 15/1096
C40B 40/08
Applicants: シムフォゲン・アクティーゼルスカブ; デンマーク、デーコー-2800コンゲンス・リングビー、ビルディング375、エレクトロヴァイ
Inventors: マルティン・ベー・オレクシウィッツ
ラース・エス・ニールセン
ペーター・エス・アナセン
マルギット・ホ・ハンセン
Priority Data: 200301867 17.12.2003 DK
200400782 15.05.2004 DK
50445503P 18.09.2003 US
50458903P 18.09.2003 US
DK2004000633 17.09.2004 WO
Title: (JA) 目的とする配列を連結するための方法
Abstract:
(JA)


多重オーバーラップ・エクステンションRT-PCRは、単一反応でヘテロメリックタンパク質のドメインまたはサブユニットをコードする2つもしくはそれ以上のヌクレオチド配列を連結する効率的な方法を提供する。
特に、例えば、免疫グロブリン、T細胞受容体、またはB細胞受容体からの可変領域コード配列の連関は、本発明の方法で容易にされる。
これは可変領域コード配列のライブラリーを生成するより効率的な方法を可能にする。
単離単一細胞由来のテンプレートを使用する多重オーバーラップ・エクステンションRT-PCRを実行する機能は、ハイスループットフォーマットで同族対合ライブラリーの生成を可能にする。


Also published as:
IS8415NO20061373MXPA/a/2006/002894KR1020060092218ZA2006/03032IL173688
EP1670912EP1921144JP2007505611RU02392324US20070141048CN1856571
CA2539576NZ545936DK1670912AU2004286019IN891/KOLNP/2006WO/2005/042774