JP2021184730 PREPARATION METHOD OF UNIT DNA COMPOSITION, AND MANUFACTURING METHOD OF DNA CONNECTED BODY

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Application Number 2021128402

Application Date 04.08.2021

Publication Number 2021184730

Publication Date 09.12.2021

Publication Kind A

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Applicants SYNPLOGEN INC
株式会社シンプロジェン

Inventors TSUGE KENJI
柘植　謙爾
ITAYA MITSUYASU
板谷　光泰

Agents 山本　秀策
森下　夏樹
▲駒▼谷　剛志
飯田　貴敏
石川　大輔
山本　健策

Priority Data 2014008690 21.01.2014 JP

Title

(EN) PREPARATION METHOD OF UNIT DNA COMPOSITION, AND MANUFACTURING METHOD OF DNA CONNECTED BODY

(JA) 単位ＤＮＡ組成物の調製方法及びＤＮＡ連結体の作製方法

Abstract

(EN)

PROBLEM TO BE SOLVED: To provide a preparation method of a unit DNA composition in which the mole number of a plurality of unit DNA are arrayed better, and a manufacturing method of a DNA connected body.

SOLUTION: A preparation method of a unit DNA composition has: a process of preparing solution including a plurality of unit DNA, to which an additional sequence is connected, for each kind of the unit DNA; a process of, after preparation of each solution, measuring the concentration of the unit DNA in each solution in the state where an additional sequence is connected to the unit DNA, dispensing each solution on the basis of its result, and making the mole numbers of the unit DNA in each solution become closer to the same with each other. A manufacturing method of a DNA connected body has: a process of preparing a unit DNA composition; a process of preparing a vector DNA; a process of removing each additional sequence from the unit DNA in which an additional sequence in solution after preparation is connected by using restriction enzyme; and a process of connecting the vector DNA and each unit DNA with each other after the removal process.

SELECTED DRAWING: None

(JA)

【課題】複数の単位ＤＮＡのモル数がより揃えられた単位ＤＮＡ組成物の調製方法及びＤＮＡ連結体の作製方法を提供すること。
【解決手段】単位ＤＮＡ組成物の調製方法は、付加配列が連結された複数の単位ＤＮＡを該単位ＤＮＡの種類毎に含む溶液を準備する工程と、各々の溶液の準備後、単位ＤＮＡに付加配列が連結された状態で、各々の溶液中の単位ＤＮＡの濃度を測定し、その結果に基づいて、各々の溶液を分取して、各々の溶液中の単位ＤＮＡのモル数を互いに同一に近づける工程と、を有する。ＤＮＡ連結体の作製方法は、単位ＤＮＡ組成物を調製する工程と、ベクターＤＮＡを準備する工程と、調製後の溶液中の付加配列が連結された単位ＤＮＡから制限酵素を用いて各々の付加配列を除去する工程と、除去工程後、ベクターＤＮＡ及び各々の単位ＤＮＡを互いに連結する工程とを有する。
【選択図】なし

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68	involving nucleic acids
6806	Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68	involving nucleic acids
6811	Selection methods for production or design of target specific oligonucleotides or binding molecules

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
34	involving hydrolase

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68	involving nucleic acids
6806	Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68	involving nucleic acids
6811	Selection methods for production or design of target specific oligonucleotides or binding molecules

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1. JP2021184730 - PREPARATION METHOD OF UNIT DNA COMPOSITION, AND MANUFACTURING METHOD OF DNA CONNECTED BODY

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
10	Processes for the isolation, preparation or purification of DNA or RNA