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PROBLEM TO BE SOLVED: To provide methods for preparing a DNA unit composition in which the mol number of a plurality of DNA units is more uniform, and to provide methods for creating concatenated DNA.
SOLUTION: The method for preparing a DNA unit composition has: a step of preparing a solution which contains a plurality of DNA units to which an added sequence is linked for each type of DNA unit; and a step of, after preparing each of the solutions, measuring the concentration of the DNA unit in each of the solutions in a state where the added sequence is linked to the DNA unit, and on the basis of the results thereof, fractionating each of the solutions and making the mol number of the DNA unit in each of the solutions closer to being identical to one another. The method for creating a concatenated DNA has: a step of preparing the DNA unit composition; a step of preparing a vector DNA; a step of removing each of the added sequence from the DNA unit to which the added sequence is linked in the solution after preparation thereof, by using a restriction enzyme; and a step of, after the removal step, linking the vector DNA and each of the DNA units with one another.
SELECTED DRAWING: None
COPYRIGHT: (C)2021,JPO&INPIT
【課題】複数の単位DNAのモル数がより揃えられた単位DNA組成物の調製方法及びDNA連結体の作製方法の提供。【解決手段】単位DNA組成物の調製方法は、付加配列が連結された複数の単位DNAを該単位DNAの種類毎に含む溶液を準備する工程と、各々の溶液の準備後、単位DNAに付加配列が連結された状態で、各々の溶液中の単位DNAの濃度を測定し、その結果に基づいて、各々の溶液を分取して、各々の溶液中の単位DNAのモル数を互いに同一に近づける工程と、を有する。DNA連結体の作製方法は、単位DNA組成物を調製する工程と、ベクターDNAを準備する工程と、調製後の溶液中の付加配列が連結された単位DNAから制限酵素を用いて各々の付加配列を除去する工程と、除去工程後、ベクターDNA及び各々の単位DNAを互いに連結する工程とを有する。【選択図】なし