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1. JP2020167997 - METHOD FOR PREPARING UNIT DNA COMPOSITION AND METHOD FOR PREPARING CONCATENATED DNA

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Office
Japan
Application Number 2020072755
Application Date 15.04.2020
Publication Number 2020167997
Publication Date 15.10.2020
Grant Number 6926270
Grant Date 06.08.2021
Publication Kind B2
IPC
C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/63
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C40B 40/06
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40Libraries per se, e.g. arrays, mixtures
04Libraries containing only organic compounds
06Libraries containing nucleotides or polynucleotides, or derivatives thereof
CPC
C12N 15/10
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/64
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
C12N 15/66
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Applicants SYNPLOGEN INC
株式会社シンプロジェン
Inventors TSUGE KENJI
柘植　謙爾
ITAYA MITSUYASU
板谷　光泰
Agents 山本　秀策
森下　夏樹
▲駒▼谷　剛志
飯田　貴敏
石川　大輔
山本　健策
Priority Data 2014008690 21.01.2014 JP
Title
(EN) METHOD FOR PREPARING UNIT DNA COMPOSITION AND METHOD FOR PREPARING CONCATENATED DNA
(JA) 単位ＤＮＡ組成物の調製方法及びＤＮＡ連結体の作製方法
Abstract
(EN)

PROBLEM TO BE SOLVED: To provide methods for preparing a DNA unit composition in which the mol number of a plurality of DNA units is more uniform, and to provide methods for creating concatenated DNA.

SOLUTION: The method for preparing a DNA unit composition has: a step of preparing a solution which contains a plurality of DNA units to which an added sequence is linked for each type of DNA unit; and a step of, after preparing each of the solutions, measuring the concentration of the DNA unit in each of the solutions in a state where the added sequence is linked to the DNA unit, and on the basis of the results thereof, fractionating each of the solutions and making the mol number of the DNA unit in each of the solutions closer to being identical to one another. The method for creating a concatenated DNA has: a step of preparing the DNA unit composition; a step of preparing a vector DNA; a step of removing each of the added sequence from the DNA unit to which the added sequence is linked in the solution after preparation thereof, by using a restriction enzyme; and a step of, after the removal step, linking the vector DNA and each of the DNA units with one another.

SELECTED DRAWING: None

COPYRIGHT: (C)2021,JPO&INPIT


(JA)

【課題】複数の単位ＤＮＡのモル数がより揃えられた単位ＤＮＡ組成物の調製方法及びＤＮＡ連結体の作製方法の提供。
【解決手段】単位ＤＮＡ組成物の調製方法は、付加配列が連結された複数の単位ＤＮＡを該単位ＤＮＡの種類毎に含む溶液を準備する工程と、各々の溶液の準備後、単位ＤＮＡに付加配列が連結された状態で、各々の溶液中の単位ＤＮＡの濃度を測定し、その結果に基づいて、各々の溶液を分取して、各々の溶液中の単位ＤＮＡのモル数を互いに同一に近づける工程と、を有する。ＤＮＡ連結体の作製方法は、単位ＤＮＡ組成物を調製する工程と、ベクターＤＮＡを準備する工程と、調製後の溶液中の付加配列が連結された単位ＤＮＡから制限酵素を用いて各々の付加配列を除去する工程と、除去工程後、ベクターＤＮＡ及び各々の単位ＤＮＡを互いに連結する工程とを有する。
【選択図】なし
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