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1. JP2018519829 - 1,3-ブタンジオールの産生のための方法および微生物

Office Japan
Application Number 2017567799
Application Date 21.07.2016
Publication Number 2018519829
Publication Date 26.07.2018
Publication Kind A
IPC
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Applicants ガバニング カウンシル オブ ザ ユニバーシティ オブ トロント
Inventors マハデヴァン,ラドハクリシュナン
ヤクニン,アレクサンダー
ガワンド,プラティシュ
ネムル,カイラ
Agents 高岡 亮一
小田 直
岩堀 明代
高橋 香元
Priority Data 62/195,011 21.07.2015 US
Title
(JA) 1,3-ブタンジオールの産生のための方法および微生物
Abstract
(JA)

1,3−BDO経路を有する非天然由来微生物が提供される。当該微生物は1,3−BDO経路酵素として、2つのアセトアルデヒドの縮合を触媒して3−ヒドロキシブタナールを産生するアルドラーゼ、および3−ヒドロキシブタナールを1,3−BDOに還元するアルド−ケトレダクターゼ、オキシドレダクターゼ、アルデヒドレダクターゼ、またはアルコールデヒドロゲナーゼの少なくとも1種類を発現する。当該微生物はさらにアセトアルデヒドを産生するために少なくとも1種類の酵素を発現し得る。生合成工程は、アルドラーゼ系の酵素を用いて2つのアセトアルデヒド分子を3−ヒドロキシブタナールに縮合し、アルド−ケトレダクターゼ、オキシドレダクターゼ、アルデヒドレダクターゼ、またはアルコールデヒドロゲナーゼ系に属する酵素を用いて3−ヒドロキシブタナールを1,3−BDOに選択的に還元することを含む。当該工程はさらに、生合成法によってアセトアルデヒドを産生することを含むことができる。
【選択図】図2