Processing

Please wait...

Settings

Settings

Goto Application

1. JP2007105056 - METHOD FOR PRODUCING PURINE NUCLEOSIDE BY FERMENTATION METHOD

Office
Japan
Application Number 2007018268
Application Date 29.01.2007
Publication Number 2007105056
Publication Date 26.04.2007
Publication Kind A
IPC
C12P 19/32
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
32having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same-ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
C12N 15/09
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12P 19/40
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
38Nucleosides
40having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
Applicants AJINOMOTO CO INC
味の素株式会社
Inventors MATSUI YUTAKA
松井 裕
KAWASAKI HISASHI
川崎 寿
SHIMAOKA MEGUMI
島岡 恵
TAKENAKA YASUHIRO
竹中 康浩
KURAHASHI OSAMU
倉橋 修
Agents 川口 嘉之
松倉 秀実
遠山 勉
Priority Data 1997194603 18.07.1997 JP
Title
(EN) METHOD FOR PRODUCING PURINE NUCLEOSIDE BY FERMENTATION METHOD
(JA) 発酵法によるプリンヌクレオシドの製造法
Abstract
(EN)

PROBLEM TO BE SOLVED: To provide a method for producing a purine nucleoside by a fermentation method.

SOLUTION: Bacteria, belonging to the genus Escherichia, modified so that the reaction branched from a purine nucleoside biosynthesis to other metabolic products, and being a reaction catalyzed by an enzyme selected from a succinyl-adenosine monophosphate synthase and purine nucleoside phosphorylase can be blocked, and having purine nucleoside-producing abilities, are cultured in a medium to allow the purine nucleoside to be formed and accumulated, and the purine nucleoside is collected.

COPYRIGHT: (C)2007,JPO&INPIT


(JA)

【課題】発酵法によるプリヌクレオシドの優れた製造法を提供する。
【解決手段】プリンヌクレオシド生合成から分岐して他の代謝産物にいたる反応が遮断されるように改変され、当該反応が、サクシニル−アデノシンモノリン酸シンターゼ、及び、プリンヌクレオシド・フォスフォリラーゼから選ばれる酵素により触媒される反応である、プリンヌクレオシド生産能を有するエシェリヒア属細菌を培地に培養し、プリンヌクレオシドを生成蓄積せしめ、プリンヌクレオシドを回収する。
【選択図】 なし