Processing

Please wait...

Settings

Settings

Goto Application

1. JP2006238816 - METHOD FOR SEPARATING AND PURIFYING NUCLEIC ACID

Office Japan
Application Number 2005060407
Application Date 04.03.2005
Publication Number 2006238816
Publication Date 14.09.2006
Publication Kind A
IPC
C12N 15/09
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C07H 21/04
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
04with deoxyribosyl as saccharide radical
CPC
C12N 15/1006
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1006by means of a solid support carrier, e.g. particles, polymers
Applicants FUJI PHOTO FILM CO LTD
富士フイルム株式会社
Inventors TAKESHITA YUMIKO
竹下 由美子
KOMAZAWA HIROYUKI
駒澤 宏幸
WATANABE SHINICHI
渡邊 信一
Agents 高松 猛
矢澤 清純
Title
(EN) METHOD FOR SEPARATING AND PURIFYING NUCLEIC ACID
(JA) 核酸の分離精製方法
Abstract
(EN)

PROBLEM TO BE SOLVED: To provide a method for separating and purifying a nucleic acid, comprising making a solid phase surface to adsorb a sample solution containing RNA and plasmid DNA and then making the solid phase surface to desorb the nucleic acid through a washing process and the like, by which the highly pure plasmid DNA can quickly be obtained in a high yield.

SOLUTION: This method for separating and purifying the nucleic acid, comprising (1) a process for bringing a sample solution containing the nucleic acid into contact with a solid phase to make the solid phase to adsorb the nucleic acid, (2) a process for bringing a washing liquid into contact with the solid phase to wash the solid phase in a nucleic acid-adsorbed state, and (3) a process for bringing a recovering liquid into contact with the solid phase to make the solid phase to desorb the nucleic acid, is characterized in that the sample solution is prepared in processes including a process for removing precipitated ingredients and then adding a surfactant and a water-soluble organic solvent to the precipitation supernatant.

COPYRIGHT: (C)2006,JPO&NCIPI

(JA)

【課題】RNAとプラスミドDNAを含む試料溶液を固相表面に吸着させた後、洗浄等を経て脱着させて核酸を分離精製する方法において、迅速、高収量、高純度なプラスミドDNAを得る方法を提供する。
【解決手段】(1)核酸を含む試料溶液を固相に接触させて、該固相に核酸を吸着させる工程、(2)洗浄液を該固相に接触させて、核酸が吸着した状態で該固相を洗浄する工程、及び(3)回収液を該固相に接触させて、該固相から核酸を脱着させる工程を含有する核酸分離精製方法において、上記試料溶液を、沈殿成分を取り除き、該沈殿上清に界面活性剤と水溶性有機溶媒を添加する工程を含んで調製することを特徴とする核酸の分離精製方法。
【選択図】なし