JP2001215228 ウイルスの検出又は測定方法

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Application Number 2000384349

Application Date 18.12.2000

Publication Number 2001215228

Publication Date 10.08.2001

Grant Number 3623162

Grant Date 03.12.2004

Publication Kind B2

IPC

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Applicants 株式会社先端生命科学研究所

Inventors 青柳　克己
大植　千春
飯田　久美子
木村　達治
八木　慎太郎

Agents 石田　敬
鶴田　準一
福本　積
西山　雅也

Title

(JA) ウイルスの検出又は測定方法

Abstract

(EN) PROBLEM TO BE SOLVED: To easily detect or measure a virus at high sensitivity.

SOLUTION: In this measuring method, a virus antibody is measured by binding it with its probe under the presence of a surfactant having an alkyl group having 10 or more of carbon numbers, and having secondary, tertiary or quaternary amine, or a nonionic surfactant, or under the presence of the both.

(JA)

【課題】ウイルスを簡便で高感度に検出または測定す
る方法の提供。

【解決手段】ウイルスの測定方法において、炭素原子
数１０個以上のアルキル基と第２、第３もしくは第４級
アミンとを有する界面活性剤又は非イオン性界面活性
剤、あるいはこの両者の存在下で、ウイルス抗原を、そ
のプローブとの結合により測定することを特徴とする方
法。

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor
576	for hepatitis

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5	Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10	Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
02	Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21	Preparation of peptides or proteins
02	having a known sequence of two or more amino acids, e.g. glutathione

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1. JP2001215228 - ウイルスの検出又は測定方法