JP1999108932 METHOD FOR DETECTION OR MEASUREMENT OF VIRUS

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Application Number 1998218136

Application Date 31.07.1998

Publication Number 1999108932

Publication Date 23.04.1999

Grant Number 3171827

Grant Date 23.03.2001

Publication Kind B2

IPC

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Applicants TONEN CORP
株式会社先端生命科学研究所

Inventors AOYANAGI KATSUMI
青柳　克己
OUE CHIHARU
大植　千春
IIDA KUMIKO
飯田　久美子
KIMURA TATSUJI
木村　達治
YAGI SHINTARO
八木　慎太郎

Agents 石田　敬
福本　積
西山　雅也

Priority Data 1997209522 04.08.1997 JP

Title

(EN) METHOD FOR DETECTION OR MEASUREMENT OF VIRUS

(JA) ウイルスの検出又は測定方法

Abstract

(EN)

PROBLEM TO BE SOLVED: To realize a virus antigen detection method which is suitable for treating many samples by a method wherein a sample which contains a virus is treated with a treatment solution which contains one out of an anionic surface-active agent, an amphoteric ionic surface-active agent, a nonionic surface-active agent and a protein denaturant.

SOLUTION: A virus as an object to be measured is a virus which forms virus particles having a structure which is constituted of a structure protein which contains genomic RNA or DNA and of a membrane protein or a lipid membrane surrounding the protein. A biological humor such as whole blood, plasma, serum, urine, saliva, a cerebrospinal fluid or the like and a liver tissue or the like are included in a sample to be measured. When a treatment agent which contains anionic surface-active agent such as SDS or the like is added to the sample to be measured, the virus is destroyed, an anticore antibody in the sample is denatured, and a core antigen in the sample is isolated. Alternatively, when an amphoteric ionic surface-active agent or a protein denaturant which displayed an action to destroy the higher-order structure of a protein is added in addition to the anionic surface-active agent, a denaturing action with reference to the solid-phase antibody of SDS is measured, and the sensitivity of the detection or the measurement of the virus is increased.

(JA)

【課題】ウイルスを簡便で高感度に検出または測定す
る方法の提供。

【解決手段】ウイルスを含む検体を、（１）陰イオン
性界面活性剤、及び（２）両イオン性界面活性剤、非イ
オン性界面活性剤又は蛋白質変性剤のいずれかを含む処
理液で処理することを特徴とするウイルス含有検体の処
理方法；並びにこの方法の実施のためのモノクローナル
抗体及びそれを生産するハイブリドーマ。

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor
569	for microorganisms, e.g. protozoa, bacteria, viruses

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21	Preparation of peptides or proteins
08	Monoclonal antibodies

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor
531	Production of immunochemical test materials

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor
543	with an insoluble carrier for immobilising immunochemicals

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor
577	involving monoclonal antibodies

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1. JP1999108932 - METHOD FOR DETECTION OR MEASUREMENT OF VIRUS

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R	INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1	Microorganisms
91	Cell lines