JP1999051940 DETECTING/MEASURING METHOD FOR HCV

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Application Number 1997209515

Application Date 04.08.1997

Publication Number 1999051940

Publication Date 26.02.1999

Grant Number 3176570

Grant Date 06.04.2001

Publication Kind B2

IPC

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Applicants TONEN CORP
株式会社先端生命科学研究所

Inventors AOYANAGI KATSUMI
青柳　克己
OUE CHIHARU
大植　千春
KIMURA TATSUJI
木村　達治
IIDA KUMIKO
飯田　久美子
YAGI SHINTARO
八木　慎太郎

Agents 石田　敬
福本　積
戸田　利雄
西山　雅也

Title

(EN) DETECTING/MEASURING METHOD FOR HCV

(JA) ＨＣＶの検出又は測定方法

Abstract

(EN)

PROBLEM TO BE SOLVED: To simply detect and quantify HCV with high sensitivity by processing a specimen containing HCV with a processing solution containing chaotropic ions and an acidifier.

SOLUTION: The problem of acid treatment such as the formation of precipitates and the contradictory problem of guanidine treatment are resolved when acid treatment and guanidine treatment are combined. When a surfactant is added to a treating agent generating chaotropic ions such as guanidine and a treating agent constituted of an acidifier, a further favorable result is obtained. Hydrochloric acid, sulfuric acid, acetic acid, trifluoroacetic acid, and trichloroacetic acid are suitable for the acidifier. A specimen containing virus grains such as HCV is processed by these methods, and a virus antigen can be simply detected and quantified with good sensitivity by the so-called immunoassay detecting the antigen with an antibody used as a probe.

(JA)

【課題】ＨＣＶを簡便で高感度に検出または測定する
方法の提供。

【解決手段】ＨＣＶを含む検体を、（１）カオトロピ
ックイオン、及び（２）酸性化剤を含む処理液で処理す
ることを特徴とするＨＣＶ含有検体の処理方法；並びに
この方法の実施のためのモノクローナル抗体及びそれを
生産するハイブリドーマ。

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor
576	for hepatitis

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor
577	involving monoclonal antibodies

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5	Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10	Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
02	Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21	Preparation of peptides or proteins
08	Monoclonal antibodies

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