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PROBLEM TO BE SOLVED: To simply detect and quantify HCV with high sensitivity by processing a specimen containing HCV with a processing solution containing chaotropic ions and an acidifier.
SOLUTION: The problem of acid treatment such as the formation of precipitates and the contradictory problem of guanidine treatment are resolved when acid treatment and guanidine treatment are combined. When a surfactant is added to a treating agent generating chaotropic ions such as guanidine and a treating agent constituted of an acidifier, a further favorable result is obtained. Hydrochloric acid, sulfuric acid, acetic acid, trifluoroacetic acid, and trichloroacetic acid are suitable for the acidifier. A specimen containing virus grains such as HCV is processed by these methods, and a virus antigen can be simply detected and quantified with good sensitivity by the so-called immunoassay detecting the antigen with an antibody used as a probe.
COPYRIGHT: (C)1999,JPO
【課題】 HCVを簡便で高感度に検出または測定する 方法の提供。
【解決手段】 HCVを含む検体を、(1)カオトロピ ックイオン、及び(2)酸性化剤を含む処理液で処理す ることを特徴とするHCV含有検体の処理方法;並びに この方法の実施のためのモノクローナル抗体及びそれを 生産するハイブリドーマ。