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1. IN9358/DELNP/2011 - NOVEL METHOD FOR QUANTIFYING PROTEINS BY MASS SPECTROMETRY

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Office
India
Application Number 9358/DELNP/2011
Application Date 29.11.2011
Publication Number 9358/DELNP/2011
Publication Date 22.02.2013
Publication Kind A
IPC
G01N 33/68
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
Applicants BIOMERIEUX
UNIVERSITE CLAUDE BERNARD LYON I
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Inventors JEROME LEMOINE
ARNAUD SALVADOR
JEAN-PHILIPPE CHARRIER
TANGUY FORTIN
Priority Data 0953576 29.05.2009 FR
Title
(EN) NOVEL METHOD FOR QUANTIFYING PROTEINS BY MASS SPECTROMETRY
Abstract
(EN) The present invention relates to a method for the quantitative detection of a target protein in a sample, in which the second-generation fragment ions are detected for providing a series of, quantitative measurements, at least one of which is correlated to the amount of proteotypic peptide generated and to the amount of target protein in the sample, characterized in that the selected first-generation fragment ion having a mass (m/z)2 is a doubly-charged peptide having a proline and/or a histidine in position 1.
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