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1. IN1362/MUMNP/2011 - METHOD FOR DETECTING AN ANALYTE IN A SAMPLE BY MULTIPLEXING FRET ANALYSIS AND KIT

Office
India
Application Number 1362/MUMNP/2011
Application Date 30.06.2011
Publication Number 1362/MUMNP/2011
Publication Date 13.01.2012
Publication Kind A
IPC
G01N 33/542
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
542with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/58
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
Applicants UNIVERSITAT POTSDAM
CNRS
CEZANNE S.A.S.
Inventors NIKO HILDEBRANDT
DANIEL GEIBLER
HANS-GERD LOHMANNSROBEN
EMMANUEL BOIS
LOIC CHARBONNIERE
RAYMOND ZIESSEL
Priority Data 09 00 828.5-1223 22.01.2009 EP
09000828 22.01.2009 EP
Title
(EN) METHOD FOR DETECTING AN ANALYTE IN A SAMPLE BY MULTIPLEXING FRET ANALYSIS AND KIT
Abstract
(EN) The invention concerns a method for detecting an analyte in a sample by multiplexing FRET (Forster Resonance Energy Transfer) analysis, the method comprising the following steps: providing a sample containing an energy transfer donor, several spectrally different quantum dot species, and an analyte, wherein the analyte is configured to mediate an energy transfer within a first energy transfer donor-acceptor pair provided by the energy transfer donor and a first quantum dot specie, the energy transfer donor is configured to act as energy transfer do-nor in the first energy transfer donor-acceptor pair, and the first quantum dot specie is confi-gured to act as energy transfer acceptor in the first energy transfer donor-acceptor pair, irra-diating excitation light from an excitation light source to the sample, in the first energy trans-fer donor-acceptor pair, transferring excitation energy from the energy transfer donor excited by the excitation light to the first quantum dot specie, the energy transfer being mediated by the analyte, and detecting emission light emitted by the first quantum dot specie after receiv-ing the excitation energy. Ref. Fig.