(EN) The invention concerns a method for detecting an analyte in a sample by multiplexing FRET (Forster Resonance Energy Transfer) analysis, the method comprising the following steps: providing a sample containing an energy transfer donor, several spectrally different quantum dot species, and an analyte, wherein the analyte is configured to mediate an energy transfer within a first energy transfer donor-acceptor pair provided by the energy transfer donor and a first quantum dot specie, the energy transfer donor is configured to act as energy transfer do-nor in the first energy transfer donor-acceptor pair, and the first quantum dot specie is confi-gured to act as energy transfer acceptor in the first energy transfer donor-acceptor pair, irra-diating excitation light from an excitation light source to the sample, in the first energy trans-fer donor-acceptor pair, transferring excitation energy from the energy transfer donor excited by the excitation light to the first quantum dot specie, the energy transfer being mediated by the analyte, and detecting emission light emitted by the first quantum dot specie after receiv-ing the excitation energy. Ref. Fig.
