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1. (IN891/KOLNP/2006) METHOD FOR LINKING SEQUENCES OF INTEREST

Office : India
Application Number: 891/KOLNP/2006 Application Date: 10.04.2006
Publication Number: 891/KOLNP/2006 Publication Date: 13.04.2007
Grant Number: 236445 Grant Date: 26.03.2010
Publication Kind : B
Prior PCT appl.: Application Number:PCTDK2004000633 ; Publication Number:WO2005042774 Click to see the data
IPC:
C12N 15/10
C07K 16/00
C07K 16/12
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
12
against material from bacteria
Applicants: SYMPHOGEN A/S
Inventors: OLEKSIEWICZ MARTIN B
NIELSEN LARS S
ANDERSEN PETER S
HANSEN MARGIT H
Priority Data: PA200301867 17.12.2003 DK
PA200400782 15.05.2004 DK
60/504,455 18.09.2003 US
60/504,589 18.09.2003 US
Title: (EN) METHOD FOR LINKING SEQUENCES OF INTEREST
Abstract:
(EN) Multiplex overlap-extension RT-PCR provides an efficient method of linking two or more nucleotide sequences encoding for domains or subunits of a heteromeric protein, in a single reaction. Especially, the linkage of variable region encoding sequences from e.g. immunoglobulins, T cell receptors or B cell receptors is eased with the method of the present invention. This allows for a more efficient way of generating libraries of variable region encoding sequences. The capability to perform the multiplex overlap-extension RT-PCR using template derived from an isolated single cell enables the generation of cognate pair libraries in a high-throughput format.
Also published as:
IS8415NO20061373MXPA/a/2006/002894KR1020060092218ZA2006/03032IL173688
EP1670912EP1921144JP2007505611RU02392324US20070141048JP4651619
CN1856571CA2539576NZ545936DK1670912AU2004286019WO/2005/042774