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1. (IL96136) USE OF HYDROPHOBIC CHROMATOGRAPHY IN A METHOD FOR PURIFICATION OF COPPER-ZINC SUPEROXIDE DISMUTASE FROM CELLS CONTAINING SAME

Office : Israel
Application Number: 96136 Application Date: 26.10.1990
Publication Number: 96136 Publication Date: 06.12.2000
Publication Kind : B
CPC:
C12N 9/0089
Applicants: SAVIENT PHARMACEUTICALS, INC.
Inventors:
Agents: ביוטכנולוגיה כללית (ישראל) בע" מ
Priority Data: 432871 07.11.1989 US
Title: (EN) USE OF HYDROPHOBIC CHROMATOGRAPHY IN A METHOD FOR PURIFICATION OF COPPER-ZINC SUPEROXIDE DISMUTASE FROM CELLS CONTAINING SAME
(HE) שמוש בכרומטוגרפיה הידרופובית בשיטה לטיהור סופראוקסיד דיסמוטז מתאים המכילים אותו
Abstract:
(EN) A method for recovering a solution containing purified, enzymatically active Cu-Zn superoxide dismutase or a polypetide analog thereof having substantially the same amino acid sequence as, and the biological activity of, naturally-occurring Cu-Zn superoxide dismutase from a composition which comprises cells containing Cu-Zn superoxide dismutase or a polypeptide analog thereof comprising: (a) treating the composition so as to separate soluble proteins present in the cells from whole cells, cellular debris and insoluble proteins so as to obtain a solution containing such soluble proteins; (b) treating the resulting solution containing the soluble proteins with a second solution containing a salt at a concentration such that the soluble proteins other than Cu-Zn superoxide dismutase or the polypeptide analog thereof present in the solution containing the soluble proteins are rendered capable of binding to an appropriate hydrophobic substance; (c) contacting the then-resulting solution containing the soluble proteins with an appropriate hydrophobic subtance so as to bind the soluble proteins other than Cu-Zn superoxide dismutase or the polypeptide analog thereof present in such solution to the hydrophobic substance and thus separate such other proteins from the Cu- Zn superoxide dimutase or polypeptide analog thereof; and (d) recovering the resulting solution containing purified, enzymatically active Cu-Zn superoxide dismutase or polypeptide analog thereof. 1625 כ" ג בתמוז התש" ס - July 26, 2000
Also published as:
EP0499621CA2073043AU1991068718WO/1991/006634