FR2946147 NOUVEAU PROCEDE DE QUANTIFICATION DE PROTEINES PAR SPECTROMETRIE DE MASSE

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France

Application Number 0953576

Application Date 29.05.2009

Publication Number 2946147

Publication Date 03.12.2010

Grant Number 2946147

Grant Date 31.08.2012

Publication Kind B1

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Applicants BIOMERIEUX SA
UNIV CLAUDE BERNARD LYON
CENTRE NAT RECH SCIENT

Inventors LEMOINE JEROME
SALVADOR ARNAUD
CHARRIER JEAN PHILIPPE
FORTIN TANGUY

Priority Data 0953576 29.05.2009 FR

Title

(FR) NOUVEAU PROCEDE DE QUANTIFICATION DE PROTEINES PAR SPECTROMETRIE DE MASSE

Abstract

(FR) La présente invention concerne un procédé pour détecter, de manière quantitative, une protéine cible dans un échantillon, dans lequel des ions fragments de seconde génération sont détectés pour donner une série de mesures quantitatives, dont au moins une est corrélée à la quantité du peptide protéotypique généré et à la quantité de protéine cible présente dans l'échantillon, caractérisé en ce que l'ion fragment de première génération de masse (m/z) sélectionné est un peptide doublement chargé qui présente, une proline et/ou une histidine en position 1.

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68	involving proteins, peptides or amino acids

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27	Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
62	by investigating the ionisation of gases; by investigating electric discharges, e.g. emission of cathode

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30	Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02	Column chromatography
62	Detectors specially adapted therefor
72	Mass spectrometers

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68	involving proteins, peptides or amino acids

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27	Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
62	by investigating the ionisation of gases; by investigating electric discharges, e.g. emission of cathode

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1. FR2946147 - NOUVEAU PROCEDE DE QUANTIFICATION DE PROTEINES PAR SPECTROMETRIE DE MASSE

C	CHEMISTRY; METALLURGY
07	ORGANIC CHEMISTRY
K	PEPTIDES
1	General methods for the preparation of peptides ; , i.e. processes for the organic chemical preparation of peptides or proteins of any length
14	Extraction; Separation; Purification
16	by chromatography

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34	involving hydrolase
37	involving peptidase or proteinase