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1. EP2449097 - POLYPEPTIDES HAVING TYROSINASE ACTIVITY AND USES THEREOF

Office European Patent Office
Application Number 10730343
Application Date 01.07.2010
Publication Number 2449097
Publication Date 09.05.2012
Publication Kind A1
IPC
C12N 9/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
CPC
C12N 9/0059
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0055acting on diphenols and related substances as donors (1.10)
0057with oxygen as acceptor (1.10.3)
0059Catechol oxidase (1.10.3.1), i.e. tyrosinase
Applicants EMPA
Inventors THOENY-MEYER LINDA CHRISTIANE
FAIRHEAD MICHAEL
Priority Data 10232009 01.07.2009 CH
2010000170 01.07.2010 CH
Title
(DE) POLYPEPTIDE MIT TYROSINKINASEAKTIVITÄT UND IHRE VERWENDUNG
(EN) POLYPEPTIDES HAVING TYROSINASE ACTIVITY AND USES THEREOF
(FR) POLYPEPTIDES AYANT UNE ACTIVITÉ DE TYROSINASE ET LEURS UTILISATIONS
Abstract
(EN)
A mutant form of Verrucomicrobium spinosum tyrosinase as well as fragments thereof comprising the mature domain and having increased activity when compared to the pro-tyrosinase are disclosed. Further, methods for using the polypeptides are disclosed.

(FR)
L'invention porte sur une forme mutante de la tyrosinase de Verrucomicrobium spinosum, ainsi que sur des fragments de celle-ci comportant le domaine adulte et dont l'activité est plus grande par comparaison avec la pro-tyrosinase. En outre, l'invention porte sur des procédés d'utilisation des polypeptides.

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