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1. (EP2425001) COMBINATION ANTI-HIV VECTORS, TARGETING VECTORS, AND METHODS OF USE

Office : European Patent Office
Application Number: 10770370 Application Date: 29.04.2010
Publication Number: 2425001 Publication Date: 07.03.2012
Publication Kind : A4
Designated States: AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR
Prior PCT appl.: Application Number:US2010033042 ; Publication Number: Click to see the data
IPC:
C12N 15/867
A61K 39/21
C12N 15/113
C12N 15/49
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
86
Viral vectors
867
Retroviral vectors
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39
Medicinal preparations containing antigens or antibodies
12
Viral antigens
21
Retroviridae, e.g. equine infectious anemia virus
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
113
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
31
Genes encoding microbial proteins, e.g. enterotoxins
33
Genes encoding viral proteins
40
Proteins from RNA viruses, e.g. flaviviruses
48
Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus
49
Lentiviridae, e.g. immunodeficiency viruses such as HIV, visna-maedi virus, equine infectious anaemia virus
CPC:
A61K 48/0066
A61K 31/7105
A61K 38/53
C07K 14/7158
C12N 7/00
C12N 15/1138
C12N 15/86
C12N 2310/14
C12N 2310/141
C12N 2310/531
C12N 2320/31
C12N 2320/32
C12N 2740/16011
C12N 2740/16043
C12N 2740/16051
C12Y 603/02
Applicants: UNIV CALIFORNIA
Inventors: ANDERSON JOSEPH
BAUER GERHARD
NOLTA, JAN A.
Priority Data: 17441909 30.04.2009 US
2010033042 29.04.2010 US
Title: (DE) ANTI-HIV-KOMBINATIONSVEKTOREN, TARGETING-VEKTOREN UND ANWENDUNGSVERFAHREN
(EN) COMBINATION ANTI-HIV VECTORS, TARGETING VECTORS, AND METHODS OF USE
(FR) VECTEURS D'ASSOCIATION ANTI-VIH, VECTEURS DE CIBLAGE, ET PROCÉDÉS D'UTILISATION
Abstract:
(EN) Recombinant lentiviral vectors containing at least: a lentiviral backbone comprising essential lentiviral sequences for integration into a target cell genome; a nucleic acid encoding a CCR5 RNAi; and an expression control element that regulates expression of the nucleic acid encoding the CCR5 RNAi element, are provided by this invention. In an alternative aspect, the vector also contains polynucleotides encoding TRIM5 alpha and HIV TAR decoy sequences along with gene expression regulation elements such as promoters operatively linked to the polynucleotides. The vectors are combined with packaging plasmid and envelope plasmids and optionally conjugated to cell-specific targeting antibodies. Diagnostic and therapeutic methods for using the compositions are further provided herein.
(FR) L'invention porte sur des vecteurs lentiviraux recombinants contenant au moins : un squelette antiviral comprenant des séquences antivirales essentielles pour une intégration dans un génome de cellule cible ; un acide nucléique codant pour un ARNi CCR5 ; et un élément de régulation d'expression qui régule l'expression de l'acide nucléique codant pour l'élément d'ARNi CCR5. Sous un autre aspect, le vecteur contient également des polynucléotides codant pour des séquences leurres TRIM5 alpha et HIV TAR ainsi que des éléments de régulation d'expression génique tels que des promoteurs fonctionnellement liés aux polynucléotides. Les vecteurs sont combinés à un plasmide de conditionnement et des plasmides d'enveloppe et facultativement conjugués à des anticorps de ciblage spécifiques de cellule. L'invention porte également sur des procédés de diagnostic et thérapeutiques pour utiliser les compositions.
Also published as:
US20120076763WO/2010/127166