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1. (EP2389391) METHOD FOR TEMPORALLY CONTROLLING THE BIOLOGICAL ACTIVITY OF PROTEINS IN VERTEBRATES, AND APPLICATIONS THEREOF

Office : European Patent Office
Application Number: 10702081 Application Date: 22.01.2010
Publication Number: 2389391 Publication Date: 30.11.2011
Publication Kind : B1
Designated States: AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LI, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR
Prior PCT appl.: Application Number:EP2010050723 ; Publication Number: Click to see the data
IPC:
C07K 14/72
C12N 9/00
C12N 9/90
C12N 15/90
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
705
Receptors; Cell surface antigens; Cell surface determinants
72
for hormones
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
90
Isomerases (5.)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
87
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90
Stable introduction of foreign DNA into chromosome
CPC:
C07K 14/721
C07K 2319/715
C12N 9/90
Applicants: CENTRE NAT RECH SCIENT
UNIV ILLINOIS
UNIV STRASBOURG
Inventors: METZGER DANIEL
CHAMBON PIERRE
ZHAO HUIMIN
KATZENELLENBOGEN JOHN
Priority Data: 2010050723 22.01.2010 EP
14644909 22.01.2009 US
Title: (DE) VERFAHREN ZUR ZEITWEILIGEN STEUERUNG DER BIOLOGISCHEN AKTIVITÄT VON PROTEINEN IN WIRBELTIEREN, UND ANWENDUNGEN DAVON
(EN) METHOD FOR TEMPORALLY CONTROLLING THE BIOLOGICAL ACTIVITY OF PROTEINS IN VERTEBRATES, AND APPLICATIONS THEREOF
(FR) PROCÉDÉ POUR CONTRÔLER TEMPORAIREMENT L'ACTIVITÉ BIOLOGIQUE DE PROTÉINES CHEZ LES VERTÉBRÉS, ET SES APPLICATIONS
Abstract: front page image
(EN) The present invention relates to a method for tightly temporally controlling the biological activity of a protein of interest in a vertebrate, upon induction of the activity of a fusion protein comprising said protein of interest and an ERM polypeptide containing a mutated ligand binding domain of the human oestrogen receptor α, with a synthetic ligand that does not interfere with oestrogen signalling. In particular, the present invention concerns a method for generating tightly temporally-controlled targeted somatic mutations in a vertebrate, preferably a mouse, by inducing the activity of a fusion protein comprising a site- specific recombinase protein and an ERM polypeptide, with a synthetic ligand devoid of oestrogenic and anti-oestrogenic activities.
(FR) La présente invention concerne un procédé pour un contrôle temporaire ferme de l'activité biologique d'une protéine intéressante chez un vertébré, lors de l'induction de l'activité d'une protéine de fusion comprenant ladite protéine intéressante et un polypeptide ERM contenant un domaine de liaison au ligand muté du récepteur α des œstrogènes humains, celui-ci étant un ligand synthétique qui n'interfère pas avec la signalisation des œstrogènes. En particulier, la présente invention concerne un procédé pour générer des mutations somatiques ciblées avec un contrôle temporaire ferme chez un vertébré, de préférence une souris, en induisant l'activité d'une protéine de fusion comprenant une protéine recombinase régio-spécifique et un polypeptide ERM, avec un ligand synthétique dépourvu des activités œstrogénique et anti-œstrogénique.
Also published as:
US20120052570CA2749789WO/2010/084171