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1. EP3674402 - WEISSELLA VIRIDESCENS STRAIN AND USES THEREOF FOR THE SYNTHESIS OF EXOPOLYSACCHARIDES

Office
European Patent Office
Application Number 18382998
Application Date 27.12.2018
Publication Number 3674402
Publication Date 01.07.2020
Publication Kind A1
IPC
C12N 9/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
C12N 1/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
C12P 19/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
C12R 1/01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
01Bacteria or actinomycetales
CPC
C12N 9/1051
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
1048Glycosyltransferases (2.4)
1051Hexosyltransferases (2.4.1)
C12P 19/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
C12R 1/01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RPROCESSES USING MICROORGANISMS
1Processes using microorganisms
01using bacteria or actinomycetales
C12Y 204/01005
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
204Glycosyltransferases (2.4)
01Hexosyltransferases (2.4.1)
01005Dextransucrase (2.4.1.5)
Applicants SANT DALMAI S A U
Inventors PANELLA GUBAU JOSEP
SEGARRA MANZANO SILVIA
DEL CERRO FERNÁNDEZ CAROLINA
ÁLVAREZ PÉREZ BEATRIZ
NAVARRO BARRERA VERÓNICA
TORTAJADA SERRA MARTA
RAMÓN VIDAL DANIEL
Designated States
Priority Data 18382998 27.12.2018 EP
Title
(DE) WEISSELLA-VIRIDESCENS-STAMM UND VERWENDUNGEN DAVON ZUR SYNTHESE VON EXOPOLYSACCHARIDEN
(EN) WEISSELLA VIRIDESCENS STRAIN AND USES THEREOF FOR THE SYNTHESIS OF EXOPOLYSACCHARIDES
(FR) SOUCHE WEISSELLA VIRIDESCENS ET SES UTILISATIONS POUR LA SYNTHÈSE D'EXOPOLYSACCHARIDES
Abstract
(EN)
The present invention relates to a strain of the speciesWeissella viridescenswith accession number CECT 9735 that displays a significantly increased ability to synthesize exopolysaccharides (EPS) compared to other strains of the same genus in the prior art. Specifically, the strain of the invention described herein comprises a polynucleotide sequence encoding an enzyme responsible for the synthesis of said EPS, wherein said sequence is comprised in a mobile element and not in the cell genome. Furthermore, this invention relates to the EPS,per se,secreted by said cell, as well as the said EPS production method.

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