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1. EP3660144 - OSTEOBLASTS DERIVED FROM ORAL NEUROECTODERMAL STEM CELLS AND THEIR USE IN JAW REPAIR

Office European Patent Office
Application Number 18306591
Application Date 30.11.2018
Publication Number 3660144
Publication Date 03.06.2020
Publication Kind A1
IPC
C12N 5/077
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
077Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
C12N 5/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
CPC
C12N 5/0037
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0018Culture media for cell or tissue culture
0037Serum-free medium, which may still contain naturally-sourced components
C12N 5/0654
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0652Cells of skeletal and connective tissues; Mesenchyme
0654Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
C12N 2501/305
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
30Hormones
305Growth hormone [GH], aka. somatotropin
C12N 2501/91
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
90Polysaccharides
91Heparin
C12N 2502/115
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2502Coculture with; Conditioned medium produced by
11blood or immune system cells
115Platelets, megakaryocytes
Applicants HOPITAUX PARIS ASSIST PUBLIQUE
UNIV DE PARIS 5 RENE DESCARTES
UNIV PARIS DIDEROT PARIS 7
UNIV PARIS VAL DE MARNE
Inventors FOURNIER BENJAMIN
FERRE FRANÇOIS
GOGLY BURNO
NASSIF ALI
TAÏHI IHSÈNE
Designated States
Priority Data 18306591 30.11.2018 EP
Title
(DE) OSTEOBLASTEN, DIE AUS ORALEN NEUROECTODERMALEN STAMMZELLEN GEWONNEN WERDEN, UND DEREN VERWENDUNG BEI DER KIEFERREPARATUR
(EN) OSTEOBLASTS DERIVED FROM ORAL NEUROECTODERMAL STEM CELLS AND THEIR USE IN JAW REPAIR
(FR) OSTÉOBLASTES DÉRIVÉES DE CELLULES SOUCHES NEUROECTODERMIQUES ORALES ET LEUR UTILISATION DANS LA RÉPARATION DE MÂCHOIRES
Abstract
(EN)
The present invention concerns a method for inducing differentiation of neuroectodermal oral stem cells, in particular from gingival tissue (GSCs), into osteoblasts by culturing them in an optimal serum-free medium supplemented by necessary components such as platelet lysate, growth hormone, heparin, and/or growth factors. The invention method provides osteoblasts for cell therapy, particularly for the restoration of bone defects in maxillary bones.

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