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1. EP3562930 - MICROBIAL DETECTION DEVICES INCLUDING ADHESIVE MASKED NUTRIENTS AND METHODS OF USING THE SAME

Office
European Patent Office
Application Number 17832160
Application Date 22.12.2017
Publication Number 3562930
Publication Date 06.11.2019
Publication Kind A1
IPC
C12M 1/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
12with sterilisation, filtration, or dialysis means
C12Q 1/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
CPC
C12M 23/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
23Constructional details, e.g. recesses, hinges
02Form or structure of the vessel
04Flat or tray type, drawers
C12M 25/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
25Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
02Membranes; Filters
C12Q 1/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Applicants 3M INNOVATIVE PROPERTIES CO
Inventors YOUNG ALEXI J
BRUTINEL EVAN D
Designated States
Priority Data 201662439671 28.12.2016 US
Title
(DE) MIKROBIELLE DETEKTIONSVORRICHTUNGEN MIT HAFTENDEN MASKIERTEN NÄHRSTOFFEN UND VERFAHREN ZUR VERWENDUNG DAVON
(EN) MICROBIAL DETECTION DEVICES INCLUDING ADHESIVE MASKED NUTRIENTS AND METHODS OF USING THE SAME
(FR) DISPOSITIFS DE DÉTECTION MICROBIENNE COMPRENANT DES NUTRIMENTS MASQUÉS ADHÉSIFS ET PROCÉDÉS POUR LES UTILISER
Abstract
(EN)
Devices for microbial detection of microorganisms are provided including a body member including a substrate having a first major surface and a second major surface. The device further includes a substantially dry, first microbial growth nutrient composition disposed on a portion of the first major surface, a first adhesive composition adhered to the first microbial growth nutrient composition, and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition. The device also includes a cover sheet attached to the body member, where the cover sheet includes a first major surface facing the body member. Devices including a water-proof pouch are also provided. Methods for detecting and enumerating at least one microorganism in a sample are additionally provided, using the devices.

(FR)
La présente invention concerne des dispositifs de détection microbienne de micro-organismes comprenant un élément formant corps comprenant un substrat ayant une première surface majeure et une seconde surface majeure. Le dispositif comprend en outre une première composition de nutriment de croissance microbienne, sensiblement sèche disposée sur une partie de la première surface majeure, une première composition adhésive qui adhère à la première composition de nutriment de croissance microbienne, et une première composition formant hydrogel soluble dans l’eau froide qui adhère à la première composition adhésive. Le dispositif comprend également une feuille de recouvrement fixée à l’élément formant corps, où la feuille de recouvrement comprend une première surface majeure faisant face à l’élément formant corps. La présente invention concerne également des dispositifs comprenant une poche imperméable à l’eau. L’invention concerne également des procédés de détection et de numération d’au moins un micro-organisme dans un échantillon, en utilisant les dispositifs.