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1. EP3461559 - MANUAL OR ELECTRONIC PIPETTE DRIVEN WELL PLATE FOR NANO-LITER DROPLET STORAGE AND METHODS OF USING SAME

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[ EN ]
Claims

1. A pipette driven well plate for nano-liter droplet storage, the pipette driven well plate comprising:

an inlet;

an outlet;

a bypass channel; and

a fluidic trap containing a valve covered by a removable covering,

wherein fluid enters the fluidic trap when the removable covering is removed and the fluid enters either the fluidic trap or the bypass channel, depending on which of the fluidic trap or bypass channel has the lower hydrodynamic resistance.
  2. The pipette driven well plate of claim 1, wherein the bypass channel is cleaned by uncovering the valve by removing the covering and introducing a suction at the inlet.
  3. The pipette driven well plate of claim 2, wherein a crescent-shaped amount of fluid is formed when the suction is introduced at the inlet.
  4. The pipette driven well plate of claim 2, wherein a pipette produces the suction at the inlet.
  5. The pipette driven well plate of claim 1, wherein the bypass channel is cleaned by covering the valve with the covering and introducing cleaning fluid at the inlet.
  6. The pipette driven well plate of claim 1, wherein the fluid enters the bypass channel when the valve is covered by the removable covering and hydrodynamic resistance of the bypass channel is less than hydrodynamic resistance of the fluidic trap.
  7. The pipette driven well plate of claim 1, further comprising a constriction piping for air between the fluidic trap and the atmosphere.
  8. The pipette driven well plate of claim 1, further comprising an extended channel between the inlet and the fluidic trap.
  9. The pipette driven well plate of claim 1, wherein a second fluid is introduced at the inlet when the valve is uncovered, and wherein the first fluid within the fluidic trap is diluted by the second fluid.
  10. The pipette driven well plate of claim 9, wherein the second fluid is removed when suction is introduced at the inlet.
  11. The pipette driven well plate of claim 1, wherein the fluidic trap has a side arm and a side arm outlet, wherein when the side arm is closed, the fluidic trap traps fluid and wherein when the side arm is opened, the fluid trapped within the fluidic trap is extracted via the side arm outlet.
  12. The pipette driven well plate of claim 1, wherein the removable covering is a programmable electronic flap.
  13. The pipette driven well plate of claim 1, wherein the removable flap is a sheet of polymer.
  14. A method for using a pipette driven well plate for nano-liter droplet storage, the method comprising:

closing a valve located within a fluidic trap;

introducing a first fluid to fill the fluidic trap and partially fill a bypass channel;

opening the valve located within the fluidic trap;

removing the first fluid surrounding the valve; and

introducing a second fluid into the fluidic trap,

wherein the introducing results in a mixture of the first and second fluids.
  15. The method of claim 12, wherein the removing step further comprises:

providing a suction force at an inlet of the well plate.


  16. The method of claim 12, further comprising:

closing the valve located within the fluidic trap; and

passing cleaning fluid through the well plate.


  17. The method of claim 12, wherein the valve is opened by removing a sheet of polymer or programmable electronic flap, and wherein the valve is closed by providing a sheet of polymer or a programmable electronic flap.