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1. (EP3396428) MICROSCOPE SYSTEM
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Claims

1. A microscope system for observing a specimen via an objective lens that forms an observation image of the specimen, the microscope system comprising:

a stage on which the specimen is mounted;

one or more light sources configured to emit light irradiating the specimen;

an illumination optical system configured to irradiate the specimen with the light emitted from the light sources;

an operating unit configured to receive selection of the light sources and setting of the state and/or an amount of light of each of the light sources;

a focusing unit configured to move in a direction orthogonal to a mounting surface on which the specimen is mounted and adjust the distance between the stage and the objective lens;

an imaging unit configured to image the observation image of the specimen formed by the objective lens and generate image data of the specimen; and

a combined image generating unit configured to combine a plurality of pieces of the image data generated by the imaging unit and generate combined image data, wherein

when the combined image generating unit generates the combined image data, the microscope system enables selection of the state of optical elements constituting the illumination optical system and selection of the type, the state, and an amount of light of the light source.


  2. The microscope system according to claim 1, wherein the combined image generating unit is configured to generate the combined image data by calculating a contrast value for each pixel of the plurality of pieces of the image data generated by the imaging unit and extracting and combining pixels indicating high contrast values.
  3. The microscope system according to claim 1 or 2, further comprising a contrast map generating unit configured to generate a contrast map by combining the image data generated by the imaging unit having a luminance value with the highest contrast value for each pixel.
  4. The microscope system according to any one of claims 1 to 3, wherein the combined image generating unit is an EFI generating unit configured to generate extended focus image data.
  5. The microscope system according to any one of claims 1 to 4, wherein
the light sources are a DF ring light source and a coaxial light source, and
the combined image generating unit is configured to generate the combined image data based on the image data imaged in the state in which a lighting segment of the DF ring light source has been changed.
  6. The microscope system according to claim 5, further comprising:

an input unit configured to receive an input of an instruction signal for instructing to generate the combined image data; and

a control unit configured to control the imaging unit and the DF ring light source, wherein

when the instruction signal for instructing to generate the combined image data is input via the input unit, the control unit is configured to perform control so as to automatically change the lighting segment of the DF ring light source.


  7. The microscope system according to claim 6, wherein
the control unit includes a drive time setting unit configured to determine, based on the exposure time of the imaging unit and the transfer time of the image data, lighting time of the DF ring light source, and
the control unit is configured to perform controls, when the combined image generating unit generates the combined image data, such that a change in the lighting segment and imaging performed by the imaging unit are synchronized.
  8. The microscope system according to any one of claims 1 to 7, wherein
the illumination optical system includes an aperture stop, and
the control unit is configured to automatically perform control, when the instruction signal for instructing to generate the combined image data is input via the input unit, such that the aperture diameter of the aperture stop is set to 60 to 80% of the pupil diameter of the objective lens to be used.
  9. The microscope system according to any one of claims 5 to 8, wherein
the combined image generating unit is the EFI generating unit configured to generate extended focus image data, and
the operating unit is configured to:

receive an input of the instruction signal for instructing an amount of light of the DF ring light source and the coaxial light source, selection of an illumination mode of the DF ring light source, rotation of the lighting segment of the DF ring light source, and selection of an aperture of the aperture stop; and

select one of a mode in which extended focus image data is acquired in the state in which a change operation of an amount of light or the like of the light source performed via the operating unit is valid and a mode in which extended focus image data is acquired in the state in which a change operation of an amount of light or the like of the light source performed via the operating unit is invalid.