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1. EP3325608 - METHODS AND MICROORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL

Office European Patent Office
Application Number 16826969
Application Date 21.07.2016
Publication Number 3325608
Publication Date 30.05.2018
Publication Kind A4
IPC
C12N 1/21
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 9/00
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
C12N 9/02
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9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
C12N 9/88
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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88Lyases (4.)
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
C12N 15/53
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
53Oxidoreductases (1)
CPC
C12N 9/0006
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9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0006acting on CH-OH groups as donors (1.1)
C12N 9/88
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9Enzymes; Proenzymes; Compositions thereof
88Lyases (4.)
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
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PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
04acyclic
18polyhydric
C12P 7/24
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
24containing a carbonyl group
C12Y 101/01001
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YENZYMES
101Oxidoreductases acting on the CH-OH group of donors (1.1)
01with NAD+ or NADP+ as acceptor (1.1.1)
01001Alcohol dehydrogenase (1.1.1.1)
Applicants GOVERNING COUNCIL UNIV TORONTO
Inventors MAHADEVAN RADHAKRISHNAN
YAKUNIN ALEXANDER
GAWAND PRATISH
NEMR KAYLA
Designated States
Priority Data 201562195011 21.07.2015 US
Title
(DE) VERFAHREN UND MIKROORGANISMEN ZUR HERSTELLUNG VON 1,3-BUTANDIOL
(EN) METHODS AND MICROORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL
(FR) PROCÉDÉS ET MICRO-ORGANISMES DE PRODUCTION DE 1,3-BUTANEDIOL
Abstract
(EN)
A non-naturally occurring microorganism having a 1,3-BDO pathway is provided. The microorganism expresses at least one of the following 1,3-BDO pathway enzymes: an aldolase that catalyzes condensation of two acetaldehydes to produce 3-hydroxybutanal; and an aldo- ketoreductase, oxidoreductase, aldehyde reductase or alcohol dehydrogenase that reduces 3- hydroxybutanal to 1,3-BDO. The organism may further express one or more enzymes for producing acetaldehyde. A biosynthetic process involves condensing two acetaldehyde molecules to 3-hydroxybutanal using an enzyme from class aldolases; and selectively reducing 3-hydroxybutanal to 1,3-BDO using an enzyme belonging to the class aldo-ketoreductase, oxidoreductase, aldehyde reductase or alcohol dehydrogenase. The process can further include producing acetaldehyde by a biosynthetic method.

(FR)
La présente invention concerne un micro-organisme non d'origine naturelle ayant une voie 1,3-BDO. Le micro-organisme exprime au moins l'une des enzymes suivantes de la voie 1,3-BDO : une aldolase, qui catalyse la condensation de deux acétaldéhydes pour produire du 3-hydroxybutanal; une aldo-cétoréductase, oxydoréductase, aldéhyde réductase ou alcool déshydrogénase, qui réduit le 3-hydroxybutanal en 1,3-BDO. L'organisme peut en outre exprimer une ou plusieurs enzymes de production d'acétaldéhyde. Un procédé de synthèse biologique entraîne la condensation de deux molécules d'acétaldéhyde en 3-hydroxybutanal en utilisant une enzyme de la classe des aldolases; la réduction d'une manière sélective de 3-hydroxybutanal en 1,3-BDO en utilisant une enzyme appartenant à la classe aldo-cétoréductase, oxydoréductase, aldéhyde réductase ou alcool déshydrogénase. Le procédé peut en outre inclure la production d'acétaldéhyde par un procédé de synthèse biologique.