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1. EP2286205 - Auto-synchronous fluorescence detection method and apparatus

Office
European Patent Office
Application Number 09745297
Application Date 13.05.2009
Publication Number 2286205
Publication Date 23.02.2011
Publication Kind B1
IPC
G01N 21/64
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
G02B 21/16
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
16adapted for ultra-violet illumination
G02B 26/04
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
26Optical devices or arrangements using movable or deformable optical elements for controlling the intensity, colour, phase, polarisation or direction of light, e.g. switching, gating or modulating
02for controlling the intensity of light
04by periodically varying the intensity of light, e.g. using choppers
G02B 26/08
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
26Optical devices or arrangements using movable or deformable optical elements for controlling the intensity, colour, phase, polarisation or direction of light, e.g. switching, gating or modulating
08for controlling the direction of light
H02K 1/18
HELECTRICITY
02GENERATION, CONVERSION, OR DISTRIBUTION OF ELECTRIC POWER
KDYNAMO-ELECTRIC MACHINES
1Details of the magnetic circuit
06characterised by the shape, form or construction
12Stationary parts of the magnetic circuit
18Means for mounting or fastening magnetic stationary parts on to, or to, the stator structures
CPC
G01N 21/6458
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
645Specially adapted constructive features of fluorimeters
6456Spatial resolved fluorescence measurements; Imaging
6458Fluorescence microscopy
G01N 21/6408
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
6408with measurement of decay time, time resolved fluorescence
G01N 2021/6463
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
645Specially adapted constructive features of fluorimeters
6463Optics
G02B 21/16
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
16adapted for ultra-violet illumination ; ; Fluorescence microscopes
G02B 26/04
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
26Optical devices or arrangements using movable or deformable optical elements for controlling the intensity, colour, phase, polarisation or direction of light, e.g. switching, gating, modulating
02for controlling the intensity of light
04by periodically varying the intensity of light, e.g. using choppers
Applicants UNIV MACQUARIE
Inventors CONNALLY RUSSELL
Designated States
Priority Data 2008902350 13.05.2008 AU
Title
(DE) Verfahren und Vorrichtung für autosynchronen Fluoreszenznachweis
(EN) Auto-synchronous fluorescence detection method and apparatus
(FR) Procédé et appareil de détection de fluorescence automatiquement synchrone
Abstract
(EN) An apparatus for time-gated fluorescence or luminescence detection includes gating means (206) arranged to alternately permit light from an excitation source (242) to be directed to a sample (235) along a first communication path (231, 232), and then permit light emitted from the sample to be directed to a detector (246) along a second communication path (237, 238) while blocking the first communication path (231, 232): The gating means (206) may comprise a single chopper wheel or apertured disc, or a rotating or oscillating arm, and may further comprise one or more reflective facets (207). The gating means (206) may be driven via a magnetic rotor, with a ferrite bead placed to offset rotor magnets with respect to drive coils, when at rest, so as to assist with self starting.
(FR) L'invention porte sur un appareil de détection de fluorescence ou de luminescence à déclenchement périodique qui comprend un moyen de déclenchement (206) agencé de façon à permettre en alternance à une lumière provenant d'une source d'excitation (242) d'être dirigée vers un échantillon (235) le long d'un premier trajet de communication (231, 232), puis à permettre à la lumière émise par l'échantillon d'être dirigée vers un détecteur (246) le long d'un deuxième trajet de communication (237, 238) tout en bloquant le premier trajet de communication (231, 232). Le moyen de déclenchement (206) peut comporter un disque à ouverture ou une roue de hachage unique, ou un bras rotatif ou oscillant, et peut de plus comporter une ou plusieurs facettes réfléchissantes (207). Le moyen de déclenchement (206) peut être entraîné à l'aide d'un rotor magnétique, avec une perle de ferrite disposée de façon à décaler les aimants de rotor par rapport à des enroulements d'entraînement, au repos, de façon à aider un démarrage automatique.