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1. (EP2959774) METHOD OF CRYOPRESERVING SPERM CELLS

Office : European Patent Office
Application Number: 15177779 Application Date: 29.03.2004
Publication Number: 2959774 Publication Date: 30.12.2015
Publication Kind : B1
Designated States: AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LI, LT, LU, LV, MC, MK, NL, PL, PT, RO, SE, SI, SK, TR
Prior PCT appl.: Application Number:US2004009646 ; Publication Number: Click to see the data
IPC:
A01N 1/02
C12M 1/34
C12N 5/071
C12Q 1/04
G01N 1/30
G01N 15/00
G01N 15/14
G01N 33/48
G01N 33/487
G01N 33/50
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
N
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
1
Preservation of bodies of humans or animals, or parts thereof
02
Preservation of living parts
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
28
Preparing specimens for investigation
30
Staining; Impregnating
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15
Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15
Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10
Investigating individual particles
14
Electro-optical investigation
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
483
Physical analysis of biological material
487
of liquid biological material
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
CPC:
C12N 5/0612
A01N 1/0284
C12N 5/06
C12N 5/061
C12Q 1/02
C12Q 3/00
G01N 15/1404
G01N 15/1459
G01N 15/1468
G01N 15/147
G01N 21/63
G01N 21/6428
G01N 33/48
G01N 33/5005
G01N 2015/0065
G01N 2015/1006
G01N 2015/1406
G01N 2015/1415
G01N 2015/149
G01N 2021/6439
Y10T 436/10
Y10T 436/115831
Y10T 436/12
Y10T 436/25
Y10T 436/2525
Y10T 436/25375
Y10T 436/255
Y10T 436/2575
Applicants: INGURAN LLC
Inventors: DURACK GARY
DIDION BRADLEY
ANZAR MUHAMMAD
LUDWIG CINDY L
GRAHAM JEFFREY A
CROWLEY KATHLEEN S
Priority Data: 04749513 29.03.2004 EP
09014407 29.03.2004 EP
10184303 29.03.2004 EP
2004009646 29.03.2004 US
45860703 28.03.2003 US
45873103 28.03.2003 US
Title: (DE) VERFAHREN ZUR KRYOKONSERVIERUNG VON SAMENZELLEN
(EN) METHOD OF CRYOPRESERVING SPERM CELLS
(FR) PROCÉDÉ POUR CRYOCONSERVER DU SPERMATOZOIDES
Abstract: front page image
(EN) A method of cryopreserving sperm cells including the steps of: adjusting the concentration of the sperm cells in a solution; adding a cryoprotectant and a protein source to the sperm cells in a solution; loading the sperm cells into a container; cooling the sperm cells to a holding temperature; maintaining the sperm cells at the holding temperature for a period; cooling the sperm cells to a temperature approaching a critical temperature zone, wherein the critical temperature zone is a zone at which ice crystal formation and changes in osmotic pressure would damage the sperm cells; cooling the sperm cells through the critical temperature zone at a cooling rate that is faster than the cooling rate used to cool the sperm cells from the holding temperature to the temperature approaching the critical temperature zone; and immersing the sperm cells in liquid nitrogen.
(FR) L'invention concerne des dispositifs et des procédés permettant d'analyser des particules, y compris des dispositifs et des procédés de tri de sperme consistant : à collecter du sperme d'un animal (30) ; à sélectionner des conditions de coloration (47a) ; à colorer le sperme avec un colorant fluorescent ADN-sélectif (48) ; à trier les cellules spermatiques en fonction de la teneur en chromosomes sexuels (55) ; et à cryoconserver une population de sperme trié (61) jusqu'à son utilisation pour une insémination artificielle. Un mode de réalisation comprend un dispositif (1001) et des procédés permettant d'utiliser une pluralité d'unités de cytométrie de flux (9) partageant une plate-forme intégrée pour trier les cellules spermatiques. Dans un autre mode de réalisation, le tri cytométrique de flux consiste à utiliser les dispositifs et les procédés suivants : un embout orienteur comprenant une chicane (101) ; un système optique à éclairage vertical (109) ; à balayer par une fente les régions d'ADN localisées dans le noyau cellulaire (225) ; à réaliser un traitement numérique du signal, comprenant un échantillonnage synchrone de signaux de sortie analogiques (701), une extraction de caractéristiques de forme d'impulsion (497) d'une approximation d'une dérivée de premier ordre d'une forme d'impulsion (497) à un point de l'impulsion, ainsi que diverses stratégies de tri ; ainsi qu'un système d'étalonnage de tri automatisé (4201). Dans un autre mode de réalisation, le traitement numérique du signal consiste à échantillonner les signaux de sortie analogiques (701) à des instants données par rapport à l'émission d'impulsions par un laser éclairant. L'invention concerne également d'autres modes de réalisation sensiblement différents de ceux déjà cités, se rapportant à des étapes ou à des systèmes individuels pouvant être utilisés pour diverses applications nécessitant une analyse particulaire.
Also published as:
NZ542547MXPA/a/2005/010492EP2194379EP1608963EP2298231EP2308416
EP2308417EP2308418EP2308419EP2309245EP2309246EP2305171
EP2305172EP2305173EP2305832EP2306173EP2306174EP2308420
EP2309244EP2332492ZA2005/07151JP2006524054EP2357464JP4614947
CN1795382CA2518882DK1608963AU2004225436IN3937/DELNP/2005BR122017017790
BRPI0408857WO/2004/088283