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1. (EP1921144) Method for linking sequences of interest

Office : European Patent Office
Application Number: 08152066 Application Date: 17.09.2004
Publication Number: 1921144 Publication Date: 14.05.2008
Publication Kind : B1
Designated States: AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IT, LI, LT, LU, LV, MC, MK, NL, PL, PT, RO, SE, SI, SK, TR
Prior PCT appl.: Application Number:DK2004000633 ; Publication Number: Click to see the data
IPC:
C12N 15/10
C07K 16/00
C07K 16/12
C12N 15/13
C40B 40/08
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
12
against material from bacteria
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
12
Genes encoding animal proteins
13
Immunoglobulins
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40
Libraries per se, e.g. arrays, mixtures
04
Libraries containing only organic compounds
06
Libraries containing nucleotides or polynucleotides, or derivatives thereof
08
Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
CPC:
C07K 16/00
C07K 16/005
C07K 16/1282
C07K 2317/21
C07K 2317/55
C12N 15/1093
C12N 15/1096
C40B 40/08
Applicants: SYMPHOGEN AS
Inventors: OLEKSIEWICZ MARTIN B
NIELSEN LARS S
ANDERSEN PETER S
HANSEN MARGIT H
Priority Data: PA200301867 17.12.2003 DK
PA200400782 15.05.2004 DK
04762853 17.09.2004 EP
50445503 18.09.2003 US
50458903 18.09.2003 US
Title: (DE) Methode zur Verbindung von besonderen Sequenzen
(EN) Method for linking sequences of interest
(FR) Procedé de liaison de sequences nucleotidiques d'interet
Abstract: front page image
(EN) Multiplex overlap-extension RT-PCR provides an efficient method of linking two or more nucleotide sequences encoding for domains or subunits of a heteromeric protein, in a single reaction. Especially, the linkage of variable region encoding sequences from e.g. immunoglobulins, T cell receptors or B cell receptors is eased with the method of the present invention. This allows for a more efficient way of generating libraries of variable region encoding sequences. The capability to perform the multiplex overlap-extension RT-PCR using template derived from an isolated single cell enables the generation of cognate pair libraries in a high-throughput format.
(FR) L'invention concerne la possibilité de lier efficacement, par RT-PCR chevauchement-extension multiplex, deux ou plus de deux séquences nucléotidiques codant les domaines ou les sous-unités d'une protéine hétéromère, à travers une seule réaction. En particulier, le procédé de liaison décrit facilite la liaison des séquences de codage à région variable, par exemple à partir des immunoglobulines, des récepteurs de lymphocyte T ou B. Cela permet de produire plus facilement des librairies de telles séquences. La possibilité de mise en oeuvre de RT-PCR chevauchement-extension multiplex au moyen d'un gabarit dérivé d'une cellule isolée simple permet de produire des librairies de paires apparentées sous un fomrat à haut rendement.
Also published as:
IS8415NO20061373MXPA/a/2006/002894KR1020060092218ZA2006/03032IL173688
EP1670912JP2007505611RU02392324US20070141048JP4651619CN1856571
CA2539576NZ545936DK1670912AU2004286019IN891/KOLNP/2006WO/2005/042774