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1. (EP1899469) NUCLEOTIDE SEQUENCES MEDIATING PLANT MALE FERTILITY AND METHOD OF USING SAME

Office : European Patent Office
Application Number: 06785327 Application Date: 22.06.2006
Publication Number: 1899469 Publication Date: 19.03.2008
Publication Kind : A2
Designated States: AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LI, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR
Prior PCT appl.: Application Number:US2006024273 ; Publication Number: Click to see the data
IPC:
C12N 15/82
A01H 1/02
C12N 15/10
C12N 15/29
C12N 15/31
C12N 15/55
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1
Processes for modifying genotypes
02
Methods or apparatus for hybridisation; Artificial pollination
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
29
Genes encoding plant proteins, e.g. thaumatin
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
31
Genes encoding microbial proteins, e.g. enterotoxins
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
52
Genes encoding for enzymes or proenzymes
55
Hydrolases (3)
CPC:
C12N 15/8231
C07K 14/415
C12N 15/8287
C12N 15/8289
Applicants: PIONEER HI BRED INT
Inventors: ALBERTSEN MARC C
FOX TIMOTHY W
HERSHEY HOWARD P
HUFFMAN GARY A
TRIMNELL MARY
WU YONGZHONG
Priority Data: 16660905 24.06.2005 US
2006024273 22.06.2006 US
Title: (DE) MÄNNLICHE FRUCHTBARKEIT IN PFLANZEN VERMITTELNDE NUKLEOTIDSEQUENZEN UND VERFAHREN ZUR VERWENDUNG DAVON
(EN) NUCLEOTIDE SEQUENCES MEDIATING PLANT MALE FERTILITY AND METHOD OF USING SAME
(FR) SEQUENCES NUCLEOTIDIQUES MEDIATRICES DE LA FERTILITE MALE ET SON PROCEDE D'UTILISATION
Abstract:
(EN) Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants. In one such method, the homozygous recessive condition of male sterility causing alleles is maintained after crossing with a second plant, where the second plant contains a restoring transgene construct having a nucleotide sequence which reverses the homozygous condition. The restoring sequence is linked with a hemizygous sequence encoding a product inhibiting formation or function of male gametes. The maintainer plant produces only viable male gametes which do not contain the restoring transgene construct. Increase of the maintainer plant is also provided by self-fertilization, and selection for seed or plants which contain the construct.
(FR) L'invention concerne des séquences nucléotidiques médiatrices de la fertilité mâle des plantes, avec une molécule d'ADN et des séquences d'acide aminé comme formulée. L'invention concerne également l'identification des séquences de promoteurs et de leurs régions essentielles. Les séquences nucléotidiques sont utiles dans la mediation de la fertilité mâle des plantes. Dans un tel procédé, l'état récessif homozygote de la fertilité mâle générant des allèles est maintenue après croisement avec une deuxième plante, la deuxième plante contenant une construction transgène restaurée ayant une séquence nucléotidique qui inverse l'état homozygote. La séquence de restauration est liée à une séquence hémizygote codante pour un produit inhibant la formation ou le fonctionnement de gamètes mâles. La plante mainteneur produit seulement des gamètes mâles viables ne contenant pas la construction transgène restaurée. L'augmentation de la plante mainteneur est également obtenue par auto-fertilisation, et la sélection de semence ou de plantes contenant la construction.
Also published as:
MXMX/a/2008/000035EP2278019EP2295585BRPI0613133-6JP2008543341CN101248183
CA2613336AU2006262185VN25200VN18756ID048.4254IN10081/DELNP/2007
WO/2007/002267