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1. EP1858918 - NUCLEIC ACID FRAGMENTS AND SPECIFIC DETECTION METHOD BY MOLECULAR IDENTIFICATION OF DIFFERENT BACTERIA SPECIES OF THE GENUS ACINETOBACTER

Office European Patent Office
Application Number 06743586
Application Date 16.03.2006
Publication Number 1858918
Publication Date 28.11.2007
Publication Kind B1
IPC
C12N 15/11
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C07H 21/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C07K 14/195
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
C07K 14/22
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
22from Neisseriaceae (F), e.g. Acinetobacter
C12N 9/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C07K 14/195
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
C12N 9/1247
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
1241Nucleotidyltransferases (2.7.7)
1247DNA-directed RNA polymerase (2.7.7.6)
C12Q 1/689
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
689for bacteria
Applicants UNIV AIX MARSEILLE II
CENTRE NAT RECH SCIENT
Inventors LA SCOLA BERNARD
RAOULT DIDIER
Designated States
Priority Data 0502630 17.03.2005 FR
2006000588 16.03.2006 FR
Title
(DE) NUKLEINSÄUREFRAGMENTE UND SPEZIFISCHES DETEKTIONSVERFAHREN DURCH MOLEKULARIDENTIFIZIERUNG VERSCHIEDENER BAKTERIENSPEZIES DER ART ACINETOBACTER
(EN) NUCLEIC ACID FRAGMENTS AND SPECIFIC DETECTION METHOD BY MOLECULAR IDENTIFICATION OF DIFFERENT BACTERIA SPECIES OF THE GENUS ACINETOBACTER
(FR) FRAGMENTS D'ACIDES NUCLEIQUES ET METHODE DE DETECTION SPECIFIQUE PAR IDENTIFICATION MOLECULAIRE DE DIFFERENTES ESPECES DE BACTERIES DU GENRE ACINETOBACTER
Abstract
(EN)
The invention concerns complete rpoB genes, fragments of rpoB genes SEQ. ID. n° 9 and 11 to 32 and hypervariable fragments of said rpoB genes SEQ. ID. n° 33 and 35 to 56 and 77 to 100 and nucleic acid fragments of non-coding hypervariable sequences framing the rpoB gene SEQ. ID. n° 121 to 144 and 165 to 188, as well as oligonucleotides of sequences specific of a species derived from said fragments of hypervariable sequences as well as consensus oligonucleotides between different species of acinetobacter bacteria of sequences SEQ. ID. n° 1 to 8 for the following 24 species: A. calcoaceticus (genomic species 1), A.. baumannii (genomic species 2), genomic species 3, A. haemolyticus(genomic species 4), A. junii (genomic species 5), genomic species 6, A. johnsonii (genomic species 7), A. livoffii (genomic species 8), genomic species 9, genomic species 10, genomic species 11, A. radioresistens (genomic species 12), genomic species 13, genomic species 16, A. schindleri, A. ursingii, A. baylyi, A. bouvetii, A. gerneri, A. grimontii, A. tandoii, A., tjernbergiae, A. towneri, A.parvus. The invention also concerns the use of said oligonucleotides, said fragments of genes or said complete rpoB genes in a method for detecting by molecular identification said acinetobacter bacteria species

(FR)
La présente invention concerne des gènes rpoB complets, fragments de gènes rpoB SEQ. ID. n° 9 et 11 à 32 et fragments de séquences hypervariables desdits gènes rpoB SEQ. ID. n° 33 et 35 à 56 et 77 à 100 et fragments d'acide nucléique de séquences hypervariables non codants encadrant le gène rpoB de séquences SEQ. ID. n° 121 à 144 et 165 à 188, ainsi que des oligonucléotides de séquences spécifiques d'une espèce tirés desdits fragments de séquences hypervariables ainsi que des oligonucléotides consensus entre les différentes espèces de bactéries acinetobacter des séquences SEQ. ID. n° l à 8 pour les 24 espèces suivantes : A. calcoaceticus (espèce génomique 1), A.. baumannii (espèce génomique 2), espèce génomique 3, A. haemolyticus(espèce génomique 4), A. junii (espèce génomique 5), espèce génomique 6, A. johnsonii (espèce génomique 7), A. livoffii (espèce génomique 8), espèce génomique 9, espèce génomique 10, espèce génomique 11, A. radioresistens (espèce génomique 12), espèce génomique 13, espèce génomique 16, A. schindleri, A. ursingii, A. baylyi, A. bouvetii, A. gerneri, A. grimontii, A. tandoii, A., tjernbergiae, A. towneri, A.parvus. La présente invention a également pour objet l'utilisation desdits oligonucléotides, dits fragments de gènes ou dits gènes complets rpoB dans une méthode de détection par identification moléculaire desdites espèces de bactéries acinetobacter.