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1. EP1853705 - METHOD FOR SEPARATING AND PURIFYING NUCLEIC ACID

Office European Patent Office
Application Number 06728790
Application Date 02.03.2006
Publication Number 1853705
Publication Date 14.11.2007
Publication Kind A4
IPC
C12N 15/09
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
CPC
C12N 15/1006
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1006by means of a solid support carrier, e.g. particles, polymers
Applicants FUJIFILM CORP
Inventors TAKESHITA YUMIKO
KOMAZAWA HIROYUKI
WATANABE SHIN-ICHI
Priority Data 2005060407 04.03.2005 JP
Title
(DE) VERFAHREN ZUR TRENNUNG UND REINIGUNG VON NUKLEINSÄURE
(EN) METHOD FOR SEPARATING AND PURIFYING NUCLEIC ACID
(FR) PROCÉDÉ DE SÉPARATION ET D'ÉPURATION D'ACIDE NUCLÉIQUE
Abstract
(EN)
A method for separating and purifying nucleic acid, the method comprising: (1) a step of contacting a sample solution containing nucleic acid with a solid phase to adsorb the nucleic acid on the solid phase; (2) a step of contacting a washing solution with the solid phase to wash the solid phase in a state that the nucleic acid is adsorbed on the solid phase; and (3) a step of contacting a recovering solution with the solid phase to desorb the nucleic acid from the solid phase, wherein the sample solution is prepared by including a step of removing a precipitate component, and adding a surfactant and a water-soluble organic solvent to a supernatant solution of the precipitate.

(FR)
L’invention concerne un procédé de séparation et d’épuration d’acide nucléique, le procédé comprenant : (1) une phase de mise en contact d’une solution échantillon contenant un acide nucléique avec une phase solide pour adsorber l’acide nucléique sur la phase solide ; (2) une phase de mise en contact d’une solution de lavage avec la phase solide pour laver la phase solide sur laquelle l’acide nucléique est adsorbé ; et (3) une phase de mise en contact d’une solution de récupération avec la phase solide pour désorber l’acide nucléique de la phase solide, la solution échantillon étant élaborée en englobant une phase d’enlèvement d’un composant précipité, et en ajoutant un tensioactif et un solvant organique soluble dans l’eau à une solution excédentaire du précipité.