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1. (EP1670912) METHOD FOR LINKING SEQUENCES OF INTEREST

Office : European Patent Office
Application Number: 04762853 Application Date: 17.09.2004
Publication Number: 1670912 Publication Date: 21.06.2006
Publication Kind : B1
Designated States: AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IT, LI, LT, LU, LV, MC, MK, NL, PL, PT, RO, SE, SI, SK, TR
Prior PCT appl.: Application Number:DK2004000633 ; Publication Number: Click to see the data
IPC:
C12N 15/10
C07K 16/00
C07K 16/12
C12N 15/13
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
12
against material from bacteria
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
12
Genes encoding animal proteins
13
Immunoglobulins
CPC:
C07K 16/00
C07K 16/005
C07K 16/1282
C07K 2317/21
C07K 2317/55
C12N 15/1093
C12N 15/1096
C40B 40/08
Applicants: SYMPHOGEN AS
Inventors: OLEKSIEWICZ MARTIN B
NIELSEN LARS S
ANDERSEN PETER S
HANSEN MARGIT H
Priority Data: 2004000633 17.09.2004 DK
PA200301867 17.12.2003 DK
PA200400782 15.05.2004 DK
50445503 18.09.2003 US
50458903 18.09.2003 US
Title: (DE) VERFAHREN ZUR VERKNÜPFUNG INTERESSIERENDER SEQUENZEN
(EN) METHOD FOR LINKING SEQUENCES OF INTEREST
(FR) PROCEDE DE LIAISON DE SEQUENCES NUCLEOTIDIQUES D'INTERET
Abstract: front page image
(EN) Multiplex overlap-extension RT-PCR provides an efficient method of linking two or more nucleotide sequences encoding for domains or subunits of a heteromeric protein, in a single reaction. Especially, the linkage of variable region encoding sequences from e.g. immunoglobulins, T cell receptors or B cell receptors is eased with the method of the present invention. This allows for a more efficient way of generating libraries of variable region encoding sequences. The capability to perform the multiplex overlap-extension RT-PCR using template derived from an isolated single cell enables the generation of cognate pair libraries in a high-throughput format.
(FR) L'invention concerne la possibilité de lier efficacement, par RT-PCR chevauchement-extension multiplex, deux ou plus de deux séquences nucléotidiques codant les domaines ou les sous-unités d'une protéine hétéromère, à travers une seule réaction. En particulier, le procédé de liaison décrit facilite la liaison des séquences de codage à région variable, par exemple à partir des immunoglobulines, des récepteurs de lymphocyte T ou B. Cela permet de produire plus facilement des librairies de telles séquences. La possibilité de mise en oeuvre de RT-PCR chevauchement-extension multiplex au moyen d'un gabarit dérivé d'une cellule isolée simple permet de produire des librairies de paires apparentées sous un fomrat à haut rendement.
Also published as:
IS8415NO20061373MXPA/a/2006/002894KR1020060092218ZA2006/03032IL173688
EP1921144JP2007505611RU02392324US20070141048JP4651619CN1856571
CA2539576NZ545936DK1670912AU2004286019IN891/KOLNP/2006WO/2005/042774