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1. EP2896687 - METHOD FOR PRODUCING 3D CELL CULTURE

Office
European Patent Office
Application Number 13837501
Application Date 12.09.2013
Publication Number 2896687
Publication Date 22.07.2015
Publication Kind B1
IPC
C12N 5/071
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
C12N 5/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
CPC
C12N 5/0062
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0062General methods for three-dimensional culture
C12N 5/0671
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
067Hepatocytes
0671Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
C12N 2533/52
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2533Supports or coatings for cell culture, characterised by material
50Proteins
52Fibronectin; Laminin
C12N 2533/90
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2533Supports or coatings for cell culture, characterised by material
90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
Applicants UNIV OSAKA
MITSUBISHI PAPER MILLS LTD
Inventors AKASHI MITSURU
MATSUSAKI MICHIYA
MATSUZAWA ATSUSHI
Designated States
Priority Data 2012203154 14.09.2012 JP
Title
(DE) VERFAHREN ZUR HERSTELLUNG EINER 3D-ZELLKULTUR
(EN) METHOD FOR PRODUCING 3D CELL CULTURE
(FR) PROCÉDÉ DE FABRICATION DE CORPS DE CULTURE CELLULAIRE TRIDIMENSIONNELLE
Abstract
(EN) Provided is a novel method for producing a three-dimensional cell culture construct. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; culturing the seeded coated cells in a culture medium; and using at least a portion of the culture medium continuously for 5 days or more. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; and culturing the seeded coated cells in a culture medium. The culture process of the coated cells includes culturing the coated cells in 70 µL or more of the culture medium per 1 × 10 4 cells.
(FR) L'invention fournit un nouveau procédé de fabrication de corps de culture cellulaire tridimensionnelle. Plus précisément, l'invention concerne le procédé de fabrication d'un corps de culture cellulaire tridimensionnelle dans lequel au moins deux couches de cellule sont stratifiées. Ce procédé de fabrication inclut : l'ensemencement de cellules enrobées dont la surface est enrobée d'un film de revêtement d'un composant matrice extracellulaire ; la culture de ces cellules enrobées ainsi ensemencées dans un milieu de culture ; et la mise en œuvre continue pendant 5 jours ou plus d'au moins une partie dudit milieu de culture. En outre, dans le procédé de fabrication de corps de culture cellulaire à au moins deux couches de cellule stratifiées, qui inclut l'ensemencement de cellules enrobées dont la surface est enrobée d'un film de revêtement d'un composant matrice extracellulaire, et la culture de ces cellules enrobées ainsi ensemencées dans un milieu de culture, la culture desdites cellules enrobées inclut la culture desdites cellules enrobées dans un milieu d'au moins 70µL pour 1×104 cellules.
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