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1. (EP1620556) NUCLEIC ACID DETECTION

Office : European Patent Office
Application Number: 04730463 Application Date: 30.04.2004
Publication Number: 1620556 Publication Date: 01.02.2006
Publication Kind : A4
Prior PCT appl.: Application Number:CN2004000434 ; Publication Number: Click to see the data
IPC:
C12N 15/50
A61K 39/215
A61P 11/00
C12Q 1/68
C12Q 1/70
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
31
Genes encoding microbial proteins, e.g. enterotoxins
33
Genes encoding viral proteins
40
Proteins from RNA viruses, e.g. flaviviruses
50
Coronaviridae, e.g. infectious bronchitis virus, transmissible gastroenteritis virus
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39
Medicinal preparations containing antigens or antibodies
12
Viral antigens
215
Coronaviridae, e.g. avian infectious bronchitis virus
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
P
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
11
Drugs for disorders of the respiratory system
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
70
involving virus or bacteriophage
CPC:
C12Q 1/701
C12Q 1/686
Applicants: HAI KANG LIFE CORP LTD
Inventors: YU CHEUNG HOI
LAU LOK TING
Priority Data: 2004000434 30.04.2004 CN
0310181 02.05.2003 GB
Title: (DE) NUKLEINSÄURENACHWEIS
(EN) NUCLEIC ACID DETECTION
(FR) DETECTION D'ACIDE NUCLEIQUE
Abstract:
(EN) The present invention relates to a process for the detection of nucleic acids from biological or environmental samples. The technique involves the amplification of the nucleic acid of interest, after conversion to cDNA if necessary, after which the amplified nucleic acid is used as the template for further amplification by the real-time PCR (RT-PCR) technique. This combination of amplification procedures increases the sensitivity and specificity of the amplification. compared with conventional amplification techniques. The invention also relates to primers for the use in this method and a diagnostic test kit.
(FR) L'invention concerne un procédé pour détecter des acides nucléiques dans des échantillons biologiques ou environnementaux. Ce procédé consiste à amplifier l'acide nucléique recherché, après conversion en ADNc si nécessaire, l'acide nucléique amplifié étant alors utilisé comme matrice pour une autre amplification selon le procédé PCR (RT-PCR) en temps réel. Cette combinaison de processus d'amplification augmente la sensibilité et la spécificité de l'amplification. comparativement aux procédés d'amplification classiques. La présente invention porte également sur des amorces servant dans ce procédé et sur un kit de test diagnostic.
Also published as:
KR1020050118738JP2006524996US20080090224JP2012080884EP2597162CN1798842
GB2401175KR1020100090730KR1020110027854KR1020120094155MYPI 20041648TH71818
IN568/DELNP/2006IN5047/DELNP/2005WO/2004/097021