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1. (EP1552017) APPARATUS AND METHODS FOR DETECTING DNA IN BIOLOGICAL SAMPLES

Office : European Patent Office
Application Number: 03783907 Application Date: 12.08.2003
Publication Number: 1552017 Publication Date: 13.07.2005
Publication Kind : B1
Prior PCT appl.: Application Number:CN2003000661 ; Publication Number: Click to see the data
IPC:
C12Q 1/68
G01N 33/53
C12M 1/00
C12M 1/34
G01N 33/543
G01N 33/553
G01N 37/00
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
551
the carrier being inorganic
553
Metal or metal coated
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
37
Details not covered by any other group of this subclass
CPC:
C12Q 1/6825
C12Q 1/6837
Y10S 435/975
Y10S 977/924
Applicants: HAI KANG LIFE CORP LTD
Inventors: YU CHEUNG HOI
LAU LOK-TING
LIN SELMA SAU WAH
CHAN DUNCAN KA-YUN
Priority Data: 0300661 12.08.2003 CN
10216928 13.08.2002 US
Title: (DE) VORRICHTUNG UND VERFAHREN ZUM NACHWEIS VON DNA IN BIOLOGISCHEN PROBEN
(EN) APPARATUS AND METHODS FOR DETECTING DNA IN BIOLOGICAL SAMPLES
(FR) APPAREIL ET PROCEDES DE DETECTION D'ADN DANS DES ECHANTILLONS BIOLOGIQUES
Abstract: front page image
(EN) Apparatus and methods are described for detecting target DNA in a biological sample using capture probes and electrically-assisted hybridization. The reaction cell is formed with an attachment surface of aluminum oxide for better thermal and physical properties, and the aluminum oxide surface is coated with anti-DIG antibody to provide a convenient attachment layer for the capture probes allowing their correct orientation, while the capture probes are formed with a DIG-label so that they attach to the surface of the cell through an anti-DIG/DIG linkage.
(FR) La présente invention se rapporte à un appareil et à des procédés permettant de détecter de l'ADN cible dans un échantillon biologique au moyen de sondes de capture et d'une hybridation par voie électrique. La cellule de réaction est dotée d'une surface de fixation d'oxyde d'aluminium permettant d'obtenir de meilleures propriétés thermiques et physiques et la surface d'oxyde d'aluminium est recouverte d'un anticorps DIG permettant de constituer une couche de fixation convenant aux sondes de capture et permettant leur orientation correcte, tandis que les sondes de capture sont dotées d'une étiquette DIG permettant leur fixation à la surface de la cellule par l'intermédiaire d'une liaison anti-DIG/DIG.
Also published as:
KR1020050053614JP2005535319CN1675375CA2495520AU2003257367DK1552017
IN572/DELNP/2005WO/2004/015138