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1. EP2856117 - TWO-DIRECTIONAL SCANNING FOR LUMINESCENCE MICROSCOPY

Office European Patent Office
Application Number 13796550
Application Date 28.05.2013
Publication Number 2856117
Publication Date 08.04.2015
Publication Kind A4
IPC
G01N 15/14
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation
G01N 21/64
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
G02B 21/16
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
16adapted for ultra-violet illumination
CPC
G01N 21/6458
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
645Specially adapted constructive features of fluorimeters
6456Spatial resolved fluorescence measurements; Imaging
6458Fluorescence microscopy
G01N 15/1456
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1456without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
G01N 21/6408
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
6408with measurement of decay time, time resolved fluorescence
G01N 21/6486
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells
G01N 2201/10
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2201Features of devices classified in G01N21/00
10Scanning
G02B 21/16
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
16adapted for ultra-violet illumination ; ; Fluorescence microscopes
Applicants UNIV MACQUARIE
Inventors JIN DAYONG
LU YIQING
PIPER JAMES AUSTIN
Designated States
Priority Data 2012902232 29.05.2012 AU
2013000559 28.05.2013 AU
Title
(DE) BIDIREKTIONALE ABTASTUNG FÜR DIE LUMINESZENZMIKROSKOPIE
(EN) TWO-DIRECTIONAL SCANNING FOR LUMINESCENCE MICROSCOPY
(FR) BALAYAGE BIDIRECTIONNEL POUR MICROSCOPIE À LUMINESCENCE
Abstract
(EN)
In one form, a two-directional scanning method for luminescence microscopy is disclosed. A series of continuous scans are performed by an interrogation wide-field relative to a first direction and a target is identified. A precise position of the target is determined in the first direction. At least one scan by the interrogation wide-field is performed relative to a second direction at or near the precise position of the target in the first direction. The two-directional scanning method produces "on-the-fly" (i.e. ex tempore or impromptu) precise localization of targets. Embodiments open up new applications for background-free or background-reduced luminescence microscopy, for example time-gated or time-resolved luminescence microscopy, in a relatively fast, higher speed or more efficient manner.

(FR)
L'invention concerne, selon un mode de réalisation, un procédé de balayage bidirectionnel pour une microscopie à luminescence. Une série de balayages continus sont mis en œuvre par un champ large d'interrogation par rapport à une première direction et une cible est identifiée. Une position précise de la cible est déterminée dans la première direction. Au moins un balayage par le champ large d'interrogation est mis en œuvre par rapport à une seconde direction dans la position précise de la cible dans la première direction ou à proximité de ladite position précise. Le procédé de balayage bidirectionnel produit une localisation précise « à la volée » (c'est-à-dire ex tempore ou de manière impromptue) des cibles. Des modes de réalisation ouvrent de nouveaux champs d'application pour la microscopie à luminescence sans fond ou à fond réduit, par exemple une microscopie à luminescence à sélection temporelle ou à résolution temporelle, d'une manière relativement rapide, à une vitesse plus élevée ou d'une manière plus efficace.