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1. (EP0859857) CATIONIC POLYACRYLATES AND POLY(ALKYL)ACRYLATES OR THE CORRESPONDING ACRYLAMIDES FOR USE IN SYNTHETIC TRANSFECTION SYSTEMS

Office : European Patent Office
Application Number: 96935575 Application Date: 25.10.1996
Publication Number: 0859857 Publication Date: 26.08.1998
Publication Kind : B1
Designated States: AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LI, LU, MC, NL, PT, SE
Prior PCT appl.: Application Number:NL1996000416 ; Publication Number: Click to see the data
IPC:
C12N 15/09
A61K 47/30
A61K 47/48
A61K 48/00
C12N 15/87
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
47
Medicinal preparations characterised by the non-active ingredients used, e.g. carriers, inert additives
30
Macromolecular compounds
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
47
Medicinal preparations characterised by the non-active ingredients used, e.g. carriers, inert additives
48
the non-active ingredient being chemically bound to the active ingredient, e.g. polymer drug conjugates
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
48
Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
87
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
CPC:
C12N 15/87
A61K 47/58
Applicants: OCTOPLUS B V
Inventors: HENNINK WILHELMUS EVERHARDUS
VAN DE WETERING PETRA
Priority Data: 95202891 25.10.1995 EP
96935575 25.10.1996 EP
9600416 25.10.1996 NL
592395 27.10.1995 US
Title: (DE) KATIONISCHE POLYACRYLATE UND POLY(ALKYL)ACRYLATE ODER DIE ENTSPRECHENDE ACRYLAMIDE ZUR VERWENDUNG IN SYNTHETISCHEN TRANSFEKTIONS-SYSTEMEN
(EN) CATIONIC POLYACRYLATES AND POLY(ALKYL)ACRYLATES OR THE CORRESPONDING ACRYLAMIDES FOR USE IN SYNTHETIC TRANSFECTION SYSTEMS
(FR) POLY(C 1-6? ALKYL)ACRYLATES ET POLYACRYLATES CATIONIQUES ET ACRYLAMIDES CORRESPONDANTS A UTILISER DANS DES SYSTEMES DE TRANSFECTION SYNTHETIQUE
Abstract: front page image
(EN) The present invention relates to a synthetic transfection or blocking system comprising as a carrier a cationic, water soluble or water dispersible polyacrylate, a polyacrylamide, a poly(C1-6 alkyl)acrylate, or a poly(C1-6 alkyl)acrylamide. In addition, it relates to a method for introducing DNA fragments in target cells, comprising contacting these DNA fragments with a polyacrylate, polyacrylamide, a poly(C1-6 alkyl)acrylate or a poly(C1-6 alkyl)acrylamide, which is at least partially substituted with cationic substituents and subsequently contacting the obtained transfection system with target cells. Finally, the invention involves the use of a polyacrylate, a polyacrylamide, a poly(C1-6 alkyl)acrylate, or a poly(C1-6 alkyl)acrylamide, which is at least partially substituted with cationic substituents as a DNA carrier system.
(FR) L'invention concerne un système de transfection synthétique ou bloquant comprenant, en tant que support, un polyacrylate, un polyacrylamide, un poly(C1-6 alkyl)acrylate ou un poly(C1-6 alkyl)acrylamide cationiques, hydrosolubles ou dispersibles dans l'eau. De plus, elle concerne un procédé d'introduction de fragments d'ADN dans des cellules cibles, qui consiste à mettre ces fragments d'ADN en contact avec un polyacrylate, un polyacrylamide, un poly(C1-6 alkyl)acrylate ou un poly(C1-6 alkyl)acrylamide, au moins partiellement substitué par des substituants cationiques, puis à mettre le système de transfection obtenu en contact avec des cellules cibles. Enfin, l'invention concerne l'utilisation d'un polyacrylate, d'un polyacrylamide, d'un poly(C1-6 alkyl)acrylate ou d'un poly(C1-6 alkyl)acrylamide, au moins partiellement substitué par des substituants cationiques en tant que système de support d'ADN.
Also published as:
US5985573JP2001508641 AU1996073422WO/1997/015680