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1. EP0820595 - PROCESS FOR DETECTING HIV-TAT

Office European Patent Office
Application Number 96909957
Application Date 12.04.1996
Publication Number 0820595
Publication Date 28.01.1998
Publication Kind A1
IPC
G01N 33/569
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/53
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
G01N 33/531
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
G01N 33/569
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
CPC
G01N 33/56988
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
56983Viruses
56988AIDS or HTLV
Applicants DKFZ KREBSFORSCHUNGSZENTRUM
Inventors KRAMMER PETER
WESTENDORP MICHAEL
FRANK RAINER
BERNDT CHRISTINA
Designated States
Priority Data 19514089 13.04.1995 DE
9600641 12.04.1996 DE
Title
(DE) NACHWEISVERFAHREN FÜR HIV-TAT
(EN) PROCESS FOR DETECTING HIV-TAT
(FR) PROCEDE DE DEPISTAGE DU VIH-TAT
Abstract
(DE)
Die Erfindung betrifft ein Verfahren zum Nachweis von HIV-TAT in einer Körperprobe, umfassend die folgenden Verfahrensschritte: (a) Entnahme einer Körperprobe und Inkubation dieser mit einem Detergens und hohem Salz, (b) Größenfraktionierung der Körperprobe von (a), und (c) Inkubation der Körperprobe von (b) mit einem Anti-HIV-TAT-Antikörper. Ferner betrifft die Erfindung einen zur Durchführung des Verfahrens geeigneten Kit.

(EN)
The invention relates to a process for detecting HIV-TAT in a body sample comprising the steps: (a) taking a body sample and incubating it with a detergent and a high salt, (b) size-fractionating the body sample from (a), and (c) incubation of the body sample from (b) with an anti-HIV-TAT antibody. The invention also relates to a kit suitable for implementing the process.

(FR)
L'invention concerne un procédé de dépistage du VIH-TAT dans un échantillon prélevé sur le corps, ledit procédé comprenant les étapes suivantes: (a) prélèvement d'un échantillon sur le corps et incubation de l'échantillon avec un détergent et un sel concentré; (b) fractionnement de la taille de l'échantillon de l'étape (a); (c) incubation de l'échantillon de l'étape (b) avec un anticorps anti-VIH-TAT. L'invention concerne en outre une trousse permettant de mettre en ÷uvre ledit procédé.