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1. (EP0559698) METHODS OF IMMOBILIZING LIPOSOMES IN GEL BEADS AND SIMILAR MATERIALS, GEL BEADS AND SIMILAR MATERIALS WITH IMMOBILIZED LIPOSOMES PREPARED BY SUCH METHODS AND USES THEREOF.

Office : European Patent Office
Application Number: 91920486 Application Date: 20.11.1991
Publication Number: 0559698 Publication Date: 15.09.1993
Publication Kind : A1
Designated States: DE, FR, GB, IT, SE
Prior PCT appl.: Application Number:SE1991000788 ; Publication Number: Click to see the data
IPC:
A 61K
A61K 8/02
B 01J
A61K 8/14
B 01J
A61K 8/25
A61K 8/64
A61K 8/65
A61K 8/73
A61K 8/92
A61K 9/127
A61K 9/16
A61K 47/36
A61Q 19/00
B01J 13/02
B01J 20/28
B01J 20/281
B01J 20/283
B01J 20/291
C12M 1/00
G01N 1/28
G01N 1/36
G01N 30/88
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
9
Medicinal preparations characterised by special physical form
10
Dispersions; Emulsions
127
Liposomes
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
8
Cosmetics or similar toilet preparations
02
characterised by special physical form
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
J
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
13
Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
02
Making microcapsules or microballoons
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
8
Cosmetics or similar toilet preparations
02
characterised by special physical form
14
Liposomes
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
J
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
20
Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
22
comprising organic material
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
8
Cosmetics or similar toilet preparations
18
characterised by the composition
19
containing inorganic ingredients
25
Silicon; Compounds thereof
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
8
Cosmetics or similar toilet preparations
18
characterised by the composition
30
containing organic compounds
64
Proteins; Peptides; Derivatives or degradation products thereof
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
8
Cosmetics or similar toilet preparations
18
characterised by the composition
30
containing organic compounds
64
Proteins; Peptides; Derivatives or degradation products thereof
65
Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
8
Cosmetics or similar toilet preparations
18
characterised by the composition
72
containing organic macromolecular compounds
73
Polysaccharides
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
8
Cosmetics or similar toilet preparations
18
characterised by the composition
92
Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
9
Medicinal preparations characterised by special physical form
10
Dispersions; Emulsions
127
Liposomes
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
9
Medicinal preparations characterised by special physical form
14
Particulate form, e.g. powders
16
Agglomerates; Granulates; Microbeadlets
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
47
Medicinal preparations characterised by the non-active ingredients used, e.g. carriers, inert additives
30
Macromolecular compounds
36
Polysaccharides; Derivatives thereof
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
Q
SPECIFIC USE OF COSMETICS OR SIMILAR TOILET PREPARATIONS
19
Preparations for care of the skin
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
J
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
13
Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
02
Making microcapsules or microballoons
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
J
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
20
Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
28
characterised by their form or physical properties
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
J
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
20
Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
281
Sorbents specially adapted for preparative, analytical or investigative chromatography
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
J
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
20
Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
281
Sorbents specially adapted for preparative, analytical or investigative chromatography
282
Porous sorbents
283
based on silica
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
J
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
20
Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
281
Sorbents specially adapted for preparative, analytical or investigative chromatography
291
Gel sorbents
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
28
Preparing specimens for investigation
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
28
Preparing specimens for investigation
36
Embedding or analogous mounting of samples
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30
Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02
Column chromatography
88
Integrated analysis systems specially adapted therefor, not covered by a single one of groups G01N30/04-G01N30/86146
CPC:
B01J 20/291
A61K 8/14
A61K 9/127
A61K 9/1652
A61Q 19/00
B01J 20/28016
B01J 20/28047
B01J 2220/54
Applicants: YANG QING
LUNDAHL PER
SANDBERG MARIA
GREIJER EVA
Inventors: YANG QING
LUNDAHL PER
SANDBERG MARIA
GREIJER EVA
Priority Data: 9003706 21.11.1990 SE
Title: (DE) VERFAHREN ZUR IMMOBILISIERUNG VON LIPOSOMEN IN GELKÜGELCHEN UND ÄHNLICHEN MATERIALIEN, DURCH SOLCHE VERFAHREN HERGESTELLTE GELKÜGELCHEN UND ÄHNLICHE MATERIALIEN MIT IMMOBILISIERTEN LIPOSOMEN SOWIE DEREN VERWENDUNG.
(EN) METHODS OF IMMOBILIZING LIPOSOMES IN GEL BEADS AND SIMILAR MATERIALS, GEL BEADS AND SIMILAR MATERIALS WITH IMMOBILIZED LIPOSOMES PREPARED BY SUCH METHODS AND USES THEREOF.
(FR) METHODES D'IMMOBILISATION DE LIPOSOMES DANS DES BILLES DE GEL ET MATERIAUX SIMILAIRES PRESENTANT DES LIPOSOMES IMMOBILISEES PREPARES PAR CES METHODES, ET LEURS UTILISATIONS.
Abstract:
(EN) This invention relates to methods of immobilizing liposomes or proteoliposomes in cross-linked or non-cross-linked materials with a three-dimensional network, like gel beads or gel particles, or beads or particles of similar materials. The liposomes are trapped within the matrix or pores of the gel or material by formation of sufficiently large liposomes from a liposome suspension or an emulsion of lipids. The methods comprise preparing a suspension of liposomes and/or proteoliposomes, or an emulsion of lipids, or lipids and protein(s), and water in organic solvent; mixing the material with the suspension or the emulsion; increasing the average size of the liposomes and/or proteoliposomes which have entered the material, or effect uptake and association of emulsified lipids [and protein(s)] in the material by evaporating the organic solvent, whereby liposomes and/or proteoliposomes are formed; and removing non-immobilized liposomes and/or proteoliposomes. The used gel beads show an immobilization capacity corresponding to at least 20 $g(m)mol of phospholipids per ml packed gel, or a total internal liposome volume of at least 50 $g(m)mol per ml gel. The invention comprises gel beads or similar materials with immobilized liposomes, proteoliposomes and/or liposomes with included or inserted drugs, substances or macromolecules, and their use for chromatographic, medical, veterinary, biotechnological, synthetic, analytical and/or cosmetic purposes.
(FR) On décrit des méthodes d'immobilisation de liposomes ou de protéoliposomes dans des matériaux réticulés ou non ayant un réseau à trois dimensions, par exemple des billes de gel ou des particules de gel, ou des billes ou des particules de matériaux similaires. Les liposomes sont rendus prisonniers dans le réseau ou les pores du gel ou du matériau par la formation de liposomes suffisamment grands à partir d'une suspension de liposomes ou d'une émulsion de lipides. Les méthodes comprennent: la préparation d'une suspension de liposomes et/ou des protéoliposomes, ou d'une émulsion de lipides, ou de lipides et de protéine(s), et d'eau dans un solvant organique; mélange du matériau avec la suspension ou l'émulsion; augmentation de la taille moyenne des liposomes et/ou des protéoliposomes qui sont passés dans le matériau, ou qui réalisent l'absorption et l'association de lipides (et de protéines(s)) émulsifiés dans le matériau par évaporation du solvant organique, avec formation de liposomes et/ou de protéoliposomes; et extraction des liposomes non immobilisés et/ou des protéoliposomes. Les billes de gel utilisées présentent une capacité d'immobilisation correspondant à au moins 20 $g(m)mol de phospholipides par ml de gel concentré, ou à un volume total interne de liposomes d'au moins 50 $g(m)mol par ml de gel. L'invention concerne des billes de gel ou des matériaux similaires avec des liposomes, protéoliposomes et/ou liposomes immobilisés, contenant des médicaments, substances ou macromolécules inclus ou introduits, ainsi que leur utilisation à des fins médicales vétérinaires, biotechnologiques, de synthèse, d'analyse et/ou cosmétiques.
Also published as:
JPH06505913AU1991089268WO/1992/009267