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1. EP2796558 - Improved gene targeting and nucleic acid carrier molecule, in particular for use in plants

Office European Patent Office
Application Number 13164966
Application Date 23.04.2013
Publication Number 2796558
Publication Date 29.10.2014
Publication Kind A1
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
C12N 15/82
CPC
C12N 15/64
C07K 14/47
C07K 2319/02
C12N 9/16
C12N 15/8213
Applicants UNIV BONN
Inventors HOMMELSHEIM CARL
FRANTZESKAKIS LAMPRINOS
ÜLKER BEKIR
Designated States
Priority Data 13164966 23.04.2013 EP
Title
(DE) Verbessertes Gen-Targeting und Nucleinsäureträgermoleküle, insbesondere zur Verwendung bei Pflanzen
(EN) Improved gene targeting and nucleic acid carrier molecule, in particular for use in plants
(FR) Ciblage génique amélioré et molécule porteuse d'acide nucléique, en particulier pour utilisation dans des plantes
Abstract
(EN)
The present invention relates to a nucleic acid carrier molecule, comprising the general formula M-S 1 -L-W-S 2 , wherein M is a first polypeptide specifically binding to a donor nucleic acid sequence to be transferred into an organelle of a cell, W is a second polypeptide specifically binding to a target nucleic acid sequence, wherein said target nucleic acid sequence is located in an organelle of a cell, L is missing or is linking group allowing M and W flexibility and semi-independence, and S 1 and S 2 independently of each other are missing or are a signal peptide sequence, and can be fused to M and W proteins either N-or C-terminally, wherein said donor nucleic acid sequence is brought into close proximity with said target nucleic acid sequence when both nucleic acid sequences are bound to said carrier molecule. The present invention furthermore relates to methods for recombinantly transforming a nucleic acid into an organelle in a cell, preferably a plant cell, employing said nucleic acid carrier molecule.