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1. (EP0499621) IMPROVED METHOD FOR PURIFICATION OF RECOMBINANT COPPER/ZINC (CU-ZN) SUPEROXIDE DISMUTASE FROM BACTERIA OR EUCARYOTIC CELLS

Office : European Patent Office
Application Number: 90917624 Application Date: 07.11.1990
Publication Number: 0499621 Publication Date: 26.08.1992
Publication Kind : B1
Prior PCT appl.: Application Number:US1990006449 ; Publication Number: Click to see the data
IPC:
C 12N
C12N 9/02
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
CPC:
C12N 9/0089
Applicants: BIO TECHNOLOGY GENERAL CORP
Inventors: BARTFELD DANIEL
LIFSHITZ RUTH
HADARY DANY
Priority Data: 43287189 07.11.1989 US
9006449 07.11.1990 US
Title: (DE) VERBESSERTES VERFAHREN ZUR REINIGUNG REKOMBINANTER KUPFER/ZINK (CU-ZN) SUPEROXIDDISMUTASE AUS BAKTERIEN ODER EUKARYOTISCHEN ZELLEN
(EN) IMPROVED METHOD FOR PURIFICATION OF RECOMBINANT COPPER/ZINC (CU-ZN) SUPEROXIDE DISMUTASE FROM BACTERIA OR EUCARYOTIC CELLS
(FR) PROCEDE AMELIORE DE PURIFICATION DE DISMUTASE RECOMBINANTE DE SUPEROXYDE DE CUIVRE/ZINC (CU-ZN) A PARTIR DE BACTERIES OU DE CELLULES EUCARYOTES
Abstract: front page image
(EN) The subject invention provides a method for recovering a solution containing purified, enzymatically active Cu-Zn superoxide dismutase or a polypeptide analog thereof having substantially the same amino acid sequence as, and the biological activity of, naturally-occurring Cu-Zn superoxide dismutase from a composition which comprises cells containing Cu-Zn superoxide dismutase or a polypeptide analog thereof. The invention also provides a method of increasing the yield of recovered solutions having an increased concentration of b isoform of an enzymatically-active polypeptide analog of Cu-Zn superoxide dismutase from a composition which comprises cells containing a, b and c isoforms of the polypeptide analog.
(FR) Un procédé permet de récupérer une solution contenant de la dismutase purifiée enzymatiquement active de superoxyde de Cu-Zn ou un analogue polypeptidique de celle-ci ayant essentiellement la même séquence d'acides aminés et la même activité biologique de la dismutase de superoxyde de Cu-Zn naturelle, à partir d'une composition qui comprend des cellules contenant de la dismutase de superoxyde de Cu-Zn ou un analogue polypeptidique de celle-ci. Ce procédé permet en outre d'augmenter le rendement d'extraction des solutions ayant une concentration accrue de l'isoforme b d'un analogue polypeptidique enzymatiquement actif de la dismutase de superoxyde de Cu-Zn à partir d'une composition qui comprend des cellules contenant des isoformes a, b et c de l'analogue polypeptidique.
Also published as:
IL96136CA2073043AU1991068718WO/1991/006634