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1. EP0328579 - HIGH RESOLUTION ELECTROPHORETIC GEL AND METHOD FOR SEPARATING SERUM PROTEINS

Office
European Patent Office
Application Number 88906539
Application Date 15.07.1988
Publication Number 0328579
Publication Date 23.08.1989
Publication Kind B1
IPC
C07K 14/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
C07K 1/26
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General processes for the preparation of peptides
14Extraction; Separation; Purification
24by electrochemical means
26Electrophoresis
G01N 27/26
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
G01N 27/447
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
CPC
G01N 27/44747
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
44704Details; Accessories
44747Composition of gel or of carrier mixture
Applicants BECKMAN INSTRUMENTS, INC.
Inventors GURSKE, WILLIAM, A.
BLESSUM, CYNTHIA, R.
CHENG, MIN-LEE
ANDRADE, MARK, S.
Designated States
Priority Data 07074744 17.07.1987 US
Title
(DE) ELEKTROPHORETISCHES GEL HOHER AUFLÖSUNG SOWIE METHODE ZUR ABSCHEIDUNG VON SERUMPROTEINEN.
(EN) HIGH RESOLUTION ELECTROPHORETIC GEL AND METHOD FOR SEPARATING SERUM PROTEINS
(FR) GEL ELECTROPHORETIQUE A HAUTE RESOLUTION ET PROCEDE DE SEPARATION DE PROTEINES DE SERUM.
Abstract
(EN) A novel agarose gel is provided for use in the high resolution electrophoretic (HRE) separation of serum proteins. The agarose gel employs a novel gel buffer which contains either hippurate, glycine, or a mixture thereof, in combination with barbital and Tris. The agarose gel of the present invention yields improved electrophoretic separation of the serum proteins in a sample whether the same gel buffer is also used as the running buffer or whether a standard barbital running buffer is used.
(FR) Un nouveau gel à base d'agarose est destiné à être utilisé dans la séparation électrophorétique à haute résolution (HRE) de protéines de sérum. Le gel à base d'agarose utilise un nouveau tampon de gel qui contient soit de l'hippurate soit de la glycine soit un mélange de ces substances, en combinaison avec du barbital et du Tris. Le gel à base d'agarose décrit dans la présente invention permet d'obtenir une meilleure séparation électrophorétique des protéines de sérum contenues dans un échantillon, qu'on utilise le même tampon de gel également comme tampon actif ou qu'on utilise un tampon de barbital actif standard.