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1. (EP2226380) Enzyme-based fed-batch technique in liquid cultures

Office : European Patent Office
Application Number: 09154440 Application Date: 05.03.2009
Publication Number: 2226380 Publication Date: 08.09.2010
Publication Kind : B1
Designated States: AL,AT,BA,BE,BG,CH,CY,CZ,DE,DK,EE,ES,FI,FR,GB,GR,HR,HU,IE,IS,IT,LI,LT,LU,LV,MC,MK,MT,NL,NO,PL,PT,RO,RS,SE,SI,SK,TR
IPC:
C12N 1/22
C12P 21/02
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
22
Processes using, or culture media containing, cellulose or hydrolysates thereof
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
CPC:
C12N 1/22
C12P 21/02
Applicants: BIOSILTA OY
Inventors: NEUBAUER PETER
NEUBAUER ANTJE
VASALA ANTTI
Priority Data: 09154440 05.03.2009 EP
Title: (DE) Fed-Batch-Technik auf Enzymbasis in Flüssigkeitskulturen
(EN) Enzyme-based fed-batch technique in liquid cultures
(FR) Technique d'écoulement continu enzymatique dans des cultures liquides
Abstract: front page image
(EN) The present invention is generally in the field of continuous and high-cell-density cultivation in laboratory- or large-scale liquid shaken cultures. More particularly it relates to a method of enzyme-based fed-batch (EnBase) for liquid microbial prokaryotic or eukaryotic cell cultivation having the possibility to manipulate the growth rate of the cultured organisms by a controlled enzymatic release of the growth-limiting substrate-monomer from substrate-polymers or substrate-oligomers.
Also published as:
EP2403936US20120045836JP2012519474CA2752262NZ594505AU2010220253
WO/2010/100239