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1. EP2211178 - Method for detecting an analyte in a sample by multiplexing FRET analysis and kit

Office
European Patent Office
Application Number 09000828
Application Date 22.01.2009
Publication Number 2211178
Publication Date 28.07.2010
Publication Kind A1
IPC
G01N 33/542
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
542with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/58
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
CPC
B82Y 15/00
BPERFORMING OPERATIONS; TRANSPORTING
82NANOTECHNOLOGY
YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
15Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
G01N 33/542
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
542with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/588
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
588with semiconductor nanocrystal label, e.g. quantum dots
Applicants UNIV POTSDAM
CNRS DELEGATION ALSACE
CEZANNE
Inventors HILDEBRANDT NIKO DR
GEISSLER DANIEL
LOEHMANNSROEBEN HANS-GERD PROF DR
CHARBONNIERE LOIC DR
ZIESSEL RAYMOND DR
BOIS EMMANUEL DR
Designated States
Priority Data 09000828 22.01.2009 EP
Title
(DE) Verfahren zur Detektion eines Analyts in einer Probe mittels Multiplex-FRET-Analyse und Kit
(EN) Method for detecting an analyte in a sample by multiplexing FRET analysis and kit
(FR) Procédé de détection d'un analyte dans un échantillon par analyse de multiplexage de transfert de l'énergie de résonance par émission de fluorescence et kit
Abstract
(EN) The invention concerns a method for detecting an analyte in a sample by multiplexing FRET (Förster Resonance Energy Transfer) analysis, the method comprising the following steps: providing a sample containing an energy transfer donor, several spectrally different quantum dot species, and an analyte, wherein the analyte is configured to mediate an energy transfer within a first energy transfer donor-acceptor pair provided by the energy transfer donor and a first quantum dot specie, the energy transfer donor is configured to act as energy transfer donor in the first energy transfer donor-acceptor pair, and the first quantum dot specie is configured to act as energy transfer acceptor in the first energy transfer donor-acceptor pair, irradiating excitation light from an excitation light source to the sample, in the first energy transfer donor-acceptor pair, transferring excitation energy from the energy transfer donor excited by the excitation light to the first quantum dot specie, the energy transfer being mediated by the analyte, and detecting emission light emitted by the first quantum dot specie after receiving the excitation energy.