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1. CN102187811 - Tomato culture medium and tomato culture method

Office
China
Application Number 201110069183.9
Application Date 21.03.2011
Publication Number 102187811
Publication Date 21.09.2011
Grant Number 102187811
Grant Date 05.09.2012
Publication Kind B
IPC
A01H 4/00
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
4Plant reproduction by tissue culture techniques
Applicants Xiamen Overseas Chinese Subtropical Plant Introduction Garden
厦门华侨亚热带植物引种园
Inventors Zhang Wenzhu
张文珠
Lin Bingying
林炳英
Lin Deqin
林德钦
Tong Qingxuan
童庆宣
Zheng Guohua
郑国华
Agents ma yingsen
厦门南强之路专利事务所 35200
Title
(EN) Tomato culture medium and tomato culture method
(ZH) 一种番茄培养基和番茄栽培方法
Abstract
(EN)
The invention relates to a tomato culture medium and a tomato culture method and relates to a tissue culture technique for plants. The method comprises the following steps: supplying a tomato blossoming and fruiting induction culture medium and a tomato bud multiplication culture medium; sowing processed tomato seeds in the tomato culture medium and culturing under the tomato growing condition, thereby acquiring tomato sterile seedlings; splicing the cotyledons of the tomato sterile seedlings into small laminas; inoculating the small laminas into an induction culture medium which is used for inducing the forming of the calluses and adventitious buds, and performing the induction culture, thus acquiring the adventitious buds of tomato; and culturing by inoculating the adventitious buds of tomato into the tomato blossoming and fruiting induction culture medium, thereby inducing the tomato plant to bud and fruit. By sterilely sowing the tomato seeds, cutting and taking the cotyledons and hypocotyls as explants and inducing the forming of the calluses and adventitious buds, the bud multiplication culture is performed and the tomato blossoms and fruits in a test tube. The culturing periods of tomato tissue culture and blossoming and fruiting in the test tube are short, the culture is free from being influenced by seasons, the tomato blossoming induction efficiency reaches 45%-60%, and the tomato fruiting induction efficiency reaches 30%-45%.

(ZH)

一种番茄培养基和番茄栽培方法,涉及植物的组织培养技术。提供番茄开花结果诱导培养基和番茄芽增殖培养基;将经过处理的番茄种子播种到番茄培养基中,于番茄生长条件下进行培养,得番茄种子无菌苗;将番茄种子无菌苗的子叶剪接成小叶片,接种于诱导愈伤组织和不定芽形成的诱导培养基中进行诱导培养,获得番茄不定芽;将番茄不定芽接种到开花结果的诱导培养基中进行培养,番茄植株长出花苞并结果。通过番茄种子无菌播种,切取子叶和胚轴为外植体,诱导愈伤组织和不定芽形成,进行芽增殖培养,并在试管内开花结果。番茄组织培养及试管内开花结果的培育周期短,不受季节影响,番茄开花诱导率达45%~60%,番茄结果诱导率达30%~45%。